VITEK 2 Compact检测耐亚胺培南鲍曼不动杆菌在庆大霉素不同药敏表型时阿米卡星的敏感性

Evaluation of Amikacin sensitivity of imipenem resistant Acinetobacter in gentamycin sensitive phenotypes when using VITEK 2 Compact

目的探讨VITEK 2 Compact全自动微生物分析系统(VITEK 2 Compact)检测亚胺培南耐药的鲍曼不动杆菌在庆大霉素不同药敏表型情况下阿米卡星敏感性试验的准确性。方法收集使用VITEK 2 Compact检测对庆大霉素不同药敏表型、阿米卡星敏感的耐亚胺培南鲍曼不动杆菌80株,以E-test法为参考方法,同时用纸片扩散(K-B)法进行复核。结果在80株耐亚胺培南的鲍曼不动杆菌中,有22株VITEK 2 Compact检测为庆大霉素、阿米卡星敏感,经E-test法和K-B法检测为庆大霉素、阿米卡星敏感,其余58株VITEK 2Compact检测为庆大霉素耐药、阿米卡星敏感,经E-test法和K-B法检测其中4株为庆大霉素耐药、阿米卡星均敏感,54株为庆大霉素、阿米卡星均耐药。本实验中E-test法和K-B法的结果均一致,标准符合率(CA)100%,VITEK 2 Compact与E-test法CA为32.5%,极严重错误率达67.5%。结论 VITEK 2 Compact检测亚胺培南耐药鲍曼不动杆菌在庆大霉素为敏感、阿米卡星为敏感时结果与E-test法及K-B法相符;当庆大霉素药敏为耐药、阿米卡星为敏感时,可能会产生结果误差,在临床可用E-test法或K-B法进行验证。

Objective To evaluate the accuracy of VITEK 2 Compact in detecting the sensitivity of imipenem resistant Acinetobacter Baumanii to Amikacin in different gentamicin sensitive phenotypes.Methods 80 strains of imipenem resistant Acinetobacter,which were sensitive to amikacin's and different sensitivity phenotypes of gentamicin under VITEK 2 Compact test were collected.The E-test method was used as the reference method,and the K-B method was used for the reexamination.Results Of the 80 imipenem resistant Acinetobacter baumannii strains,22 were detected by VITEK 2 Compact as sensitive to Amikacin and gentamicin,Amikacin sensitivity was detected by E-test and disk diffusion assay (K-B).The other 58 strains were detected by VITEK2 Compact for Amikacin sensitivity and gentamycin resistance.Detection by E-test method and paper diffusion method,4 of them were sensitive to Amikacin and 54 were resistant to Amikacin.The results of E-test method and disc diffusion method were consistent in this experiment,and the standard coincidence rate (CA) was 100%.The CA of VITEK method and E-test was 32.5%,The rate of serious error was 67.5%.Conclusion VITEK 2 Compact detection of imipenem resistant Acinetobacter Bauman in gentamicin sensitive and Amikacin sensitive results was consistent with the E-test method and KB method.When gentamicin was resistance and Amikacin was sensitive,it may produce wrong results.E-test or KB methods were used in daily work to verify the results.