超高分辨率小动物超声影像系统在大鼠腹主动脉移植模型中的应用

Application of ultra-high resolution small animal ultrasound imaging system in rat models of abdominal artery implantation

目的探讨超高分辨率小动物超声影像系统在大鼠腹主动脉移植模型中的应用价值,观察聚ε-己内酯(PCL)-肝素人工血管在体血流动力学变化。方法选取10只Wistar大鼠,应用PCL-肝素人工血管完成大鼠腹主动脉移植,建立大鼠腹主动脉移植模型。应用超高分辨率小动物超声影像系统检测大鼠体内PCL-肝素人工血管在不同时间点的管壁厚度、管腔内径及血流速度,并进行统计学分析。结果移植模型建立后1、2、3个月,PCL-肝素人工血管管壁厚度差异有统计学意义(F=16.92,P<0.01),且1个月与2个月间(P=0.01)、1个月与3个月间(P<0.01)管壁厚度差异均有统计学意义;不同时间点PCL-肝素人工血管管腔内径(F=0.08,P=0.99)及血流速速(F=0.58,P=0.59)差异均无统计学意义。建模后1个月,PCL-肝素人工血管均无动脉瘤样扩张改变;建模后3个月,瘤样扩张的PCL-肝素人工血管管腔内径明显大于未扩张者(t=2.94,P=0.03),血流速度明显低于未扩张者(t=4.28,P=0.01)。结论超高分辨率小动物超声影像系统可精确测量PCL-肝素人工血管在大鼠腹主动脉移植模型体内的血流动力学变化。

Objective To investigate the application value of ultra-high resolution small animal ultrasound imaging in rat models of abdominal artery implantation,and to observe the hemodynamic changes of (Poly[ε-caprolactone],PCL)-heparin grafts.Methods Totally 10 rats were used to establish models of abdominal artery implantation of heparin-conjugated PCL grafts.Ultra-high resolution small animal ultrasound imaging was performed to measure wall thickness,graft luminal diameter and blood flow velocity of heparin-conjugated PCL scaffold in different time points,and then statistical analysis was performed.Results The wall thicknesses of heparin-conjugated PCL scaffold were significantly different among 1,2 and 3 months after implantation ( F =16.92,P <0.01),also between 1 month and 2 months after implantation ( P =0.01),as well as between 1 month and 3 months after implantation (P <0.01).There was no statistic difference of luminal diameter (F =0.08,P =0.99) nor blood flow velocity (F =0.58,P =0.59) of heparin-conjugated PCL scaffold among different time points after implantation.One month after implantation,no aneurysm was found in heparin-conjugated PCL scaffold.However,heparin-conjugate PCL scaffold with aneurysm showed significantly bigger luminal diameter (t =2.94,P =0.03) and lower blood flow velocity (t =4.28,P =0.01) than those without aneurysm 3 months after implantation.Conclusion Ultra-high resolution small animal ultrasound imaging can be used for accurate measurement of hemodynamic changes of heparin-conjugated PCL scaffolds in rat models of abdominal artery implantation.