海南肉芝软珊瑚msh1基因的系统进化

Phylogenetic Relationships among Sarcophyton of Hainan Based on Partial Sequence of msh1

为了在分子水平上对软珊瑚进行分类和鉴定,以海南32份肉芝软珊瑚(Sarcophyton)与目前已知的Sarcophyton种msh1基因序列进行系统进化树构建.结果在msh1碱基比对中发现,海南样本msh-11在序列717位置缺失单碱基;样本msh-50在序列632和652位置插入单碱基T;其余样品未见碱基插入和缺失.在所有已知Sarcophyton种中,Sarcophyton pauciplicatum和Sarcophyton buitendijki 2个种分化时间最早;有6组其每组内Sarcophyton种msh1序列一致;海南样本msh-5与Sarcophyton cinereum和Sarcophyton crassocaule序列相同;样本Msh-6与Sarcophyton cherbonnieri亲缘关系最近.样本msh-21和msh-17与Sarcophyton buitendijki亲缘关系最近.样本msh-44与Sarcophyton boettgeri亲缘关系最近;样本msh-30和样本msh-38与Sarcophyton ehrenbergi亲缘关系最近;其中样本msh-8、msh-7、msh-12、msh-5、msh-9和msh-40与其他Sarcophyton种亲缘关系较远.

Recently,the successful identification of soft coral species has been reported by using the homologous sequence of mitochondrial protein coding gene mutation ( msh1 ).In this study the phylogenetic tree was constructed based on msh1 gene sequences from 32 Sarcophyton collected in Hainan and all the Sarcophyton species known at present.The results showed that among 737 base pairs of msh1,a single base was found to be deleted at the position of 717 in Hainan Sample msh-11,while the single base T is inserted at the position of 632 and 652 in Sample msh-50.No base insertion and deletion were found in other samples.Among all the Sarcophyton species known,Sarcophyton pauciplicatum and Sarcophyton buitendijki have the earliest differentiation time.Each of the six groups of Sarcophyton species has the same msh1 sequence and can not be distinguished from each other.Hainan Sample msh-5 has the same sequence as Sarcophyton Cinereum and Sarcophyton crasson.Sample msh-6 is closely related to Sarcophyton cherbonnieri.Sample msh-21 and msh-17 are closely related to Sarcophyton buitendijki.Sarcophyton boettgeri has the closest genetic relationship with Sample msh-44.Sarcophyton ehrenbergi has the closest genetic relationship with Sample msh-30 and msh-38.Samples msh-8,msh-7,msh-12,msh-5,msh-9 and msh-40 are distant from other Sarcophyton species known at present.