围产期孕妇B族链球菌感染的筛查方法对结果的影响

Effect of Screening Methods for Group B Streptococcus Infection in Perinatal Women on Outcomes

目的通过比较聚合酶链反应(PCR)、显色平板法两种筛查方法,探讨围产期孕妇B族链球菌(GBS)感染的合适快速检测方法。方法选取2016年5月～2018年5月我院152例常规产检的围产期孕妇,采集孕妇阴道分泌物后分别做PCR检测与显色平板法,并对结果进行质谱检测确认。比较两种方法对GBS的阳性检出率。结果 PCR检测方法的阳性检出率为6.58%(10/152),灵敏度为77.27%(17/22),显色平板法的阳性检出率11.18%(17/152),灵敏度为50.00%(10/22)。结论两种筛查方法均能检测GBS,应用显色平板法对筛查围产期孕妇的GBS感染灵敏度更高。临床中对围产期孕妇的GBS筛查采用显色平板培养的方法能提高GBS的检出率。

Objective A suitable rapid detection method for infection of group B streptococcus (GBS) in perinatal women was conducted by comparing two methods of polymerase chain reaction (PCR) and chromogenic plate method. Methods From May 2016 to May 2018, 152 cases of perinatal pregnant women in our hospital were selected. The vaginal secretions of pregnant women were collected and PCR detection and chromogenic plate method were performed respectively. The results were confirmed by mass spectrometry. The positive detection rate of GBS was compared between the two methods. Results The positive detection rate of PCR detection method was 6.58%(10/152), the sensitivity was 77.27%(17/22), the positive detection rate of chromogenic plate method was 11.18%(17/152), and the sensitivity was 50.00%(10/22). Conclusion Both screening methods can detect GBS, and the chromogenic plate method is more sensitive to screening GBS infection in perinatal women. In clinical practice, GBS screening for perinatal pregnant women can improve the detection rate of GBS by using chromogenic plate culture.