前列腺素E1对梗阻性黄疸大鼠肠黏膜损伤的保护作用

The protective effect of prostaglandin E1 on intestinal mucosa in rats with obstructive jaundice

目的研究前列腺素E1对梗阻性黄疸大鼠肠黏膜损伤的保护作用。方法将45只Wistar雄性大鼠按随机数表法分为假手术组(Sham组)、梗阻性黄疸组(OJ组)、前列腺E1组(OJ+PGE1组),每组15只,采用胆总管双重结扎建立大鼠梗阻性黄疸模型,术后第7天起,OJ+PGE1组应用前列腺素E1注射液0.1μg·kg^(-1)·d^(-1)腹腔注射治疗14 d,其他两组腹腔注射相同剂量生理盐水。3组大鼠分别于术后第21天处死,利用酶联免疫吸附法(ELISA)检测和比较3组大鼠血清内毒素、D-乳酸、TNF-α、IL-6的水平,HE染色观察比较各组大鼠回肠绒毛高度、肠黏膜厚度,免疫组织化学染色和Western-blot检测回肠组织NF-κB蛋白的表达。结果 OJ+PGE1组及OJ组大鼠血浆内毒素、D-乳酸、TNF-α、IL-6的水平表达水平高于Sham组,但OJ+PGE1组增高的程度低于OJ组,差异有统计学意义(P<0.05);与Sham组比较,OJ+PGE1组与OJ组大鼠回肠组织NF-κB蛋白表达明显升高,但OJ+PGE1组增高的程度低于OJ组,差异有统计学意义(P<0.05)。结论前列腺素E1对梗阻性黄疸大鼠肠黏膜损伤具有保护作用,其机制可能与抑制NF-κB的活化和降低炎性因子的表达有关。

Objective To study the protective effect of prostaglandin E1 on intestinal mucosal lesion in rats with obstructive jaundice.Methods A total of 45 male Wister rats were randomly divided into the sham operation group(SH group),obstructive jaundice group(OJ group)and obstructive jaundice + prostaglandin E1 treatment group(OJ+PGE1 group),with 15 rats in each group.The SH group rats were free without ligation of the common bile duct,OJ group and Group OJ+PGE1 were treated by double thread ligation of the common bile duct,postoperative 1 week,OJ+PGE1 group application of prostaglandin E1 injection(0.1 μg·kg^-1·d^-1)continuous intraperitoneal injection for 2 weeks.The other two groups were intraperitoneally injected with the same dose of normal saline.The three groups of rats were sacrificed on the twenty-first day,expression of serum endotoxin,D-lactic acid,TNF-α,IL-6 were measured by enzyme-linked immunosorbent assay(ELISA),the ileum villus height and thickness of the rats were compared by HE,the expression of nuclear factor-κB(NF-κB)protein were detected by immunohistochemical staining and Western-blot.Results The expression level of serum endotoxin,D-lactic acid,TNF-alpha and IL-6 in the OJ+PGE1 group and OJ group were higher than the Sham group,but the OJ+PGE1 group was significantly lower than the OJ group(P<0.05);compared with the Sham group,the expression of NF-κB protein in the OJ+PGE1 group and OJ group were increased significantly,but the increase in the OJ+PGE1 group was significantly lower than the OJ group(P<0.05).Conclusion Prostaglandin E1 has protective effect on intestinal mucosal injury in rats with obstructive jaundice,and its mechanism may be related to inhibiting the activation of NF-κB and decreasing the expression of inflammatory factors.