河蚬肉酶解产物解酒护肝功效

Study on the anti-alcohol and liver-protection efficacy of hydrolysate from soft tissue of Corbicula fluminea

采用体外试验法测定河蚬肉酶解产物对乙醇脱氢酶(ADH)活性的影响,采用动物试验测定灌胃河蚬肉酶解产物后小鼠醉酒时间、醒酒时间、血液乙醇浓度的变化以及小鼠肝组织中丙二醛(MDA)、甘油三酯(TG)、谷胱甘肽(GSH)等相关生化指标评价其解酒护肝的功效。体外试验表明:河蚬肉酶解产物对ADH具有激活作用,激活率为52%。动物试验表明:河蚬肉酶解产物组与阴性组比较,可显著延长小鼠的醉酒时间和缩短醒酒时间,时间延长率为69.42%,缩短率为13.40%,且能显著降低小鼠血液乙醇浓度(P<0.05)。此外,河蚬肉酶解产物能显著降低小鼠肝组织中的MDA、TG的含量(P<0.05),减缓GSH的消耗(P<0.05)。综上可知河蚬肉酶解产物具有解酒护肝的功效。

The effect of hydrolysate fromCorbicula flumineasoft tissue on the activation of alcohol dehydrogenase(ADH)in vitro was determined,and its effects of anti-alcohol and liverprotection on mice were also observed by measuring the drunk time,sober-up time,concentration of ethanol in serum,and the contents of malondialdehyde(MDA),triglyceride(TG)and glutathione(GSH)in liver tissue of mice.The results showed that hydrolysate from soft tissue of Corbicula fluminea could activate ADH in vitro with activation rate of 52%.It could also prolong the drunk time and shorten the sober-up time of drunken mice significantly,by 69.42% and 13.40%,respectively,with the comparation of the negative group.It reduced the concentration of ethanol in mice serum significantly(P<0.05)and the contents of MDA and TG in liver tissue of mice significantly(P<0.05),and slowed down the consumption of GSH in mice significantly(P<0.05).Therefore,the hydrolysate from Corbicula fluminea soft tissue could activate ADH in vitro,with the function of anti-alcohol and protecting liver in drunk mice.