益智仁缩尿功效成分的研究

Study on various components of Alpinia oxyphylla Fructus in urine-reducing effect

探究了益智仁的缩尿功效,并对其有效成分进行了分离鉴定。通过建立小鼠多尿模型,对有效部位进行缩尿活性筛选,采用代谢笼收集小鼠前2,6h内总尿量,并分别对其尿重/体重进行计算。结果表明,75%乙醇提取物乙酸乙酯萃取物对小鼠排尿的抑制效果最为明显,该部分经MCI、ODS-D柱分离,得到4种单体化合物,包括2个脂肪酸。运用NMR等波谱方法,并参考相关文献,对所得化合物进行结构分析,分别鉴定为3-甲氧基-4羟基-二苯己烷、20-丙基-β-谷甾醇、油酸、亚油酸。3-甲氧基-4羟基-二苯己烷和20-丙基-β-谷甾醇为首次从益智仁中分离得到并证实具有缩尿作用。

This research focus on the efficiency of Alpinia Oxyphylla Fructus in urine-reducing and developing the method of separating and identifying its active components.Established a mice model with diuresis to validate the Alpinia Oxyphylla Fructus’ different components and purified by varying measures in reducing urine.The total urine volume in the first 2 and 6 hours of the mice was collected by metabolic cage.The values of urine weight/body weight were calculated respectively.The result showed that 75% ethanol extract of ethyl acetate phase was the most sensitive part in urine-reducing.Four pure monomer compounds were further separate from this part by MCI and ODS-D columns,including two fatty acids.The structure of components were identified as(1)3-methoxy-4-Hydroxy-diphenylhexane,(2)20-propyl-β-sitosterol,(3)oleic acid and(4)linoleic acid respectively,by the methods of NMR and other spectroscopy technology.Compound 1 and Compound 2 were separate from Alpinia Oxyphylla Fructus and confirmed to have effects on urine-reducing for the first time.