酸性乳糖酶对奶粉中乳糖水解工艺的研究

Study on hydrolysis conditions of lactose in milk powder by acidic lactase

以乳糖水解率为评定指标,对奶粉中乳糖在酸性乳糖酶催化下的水解作用进行了研究,考察不同乳糖添加量、温度、pH和时间对奶粉中乳糖水解率的影响。先通过单因素实验初步确定水解工艺条件,在单因素实验的基础上,选取3因素3水平的响应面模型分析确定了酸性乳糖酶水解奶粉中乳糖的最佳条件为:乳糖酶添加量0.16%,温度45.31℃,pH4.95。在此条件下乳糖水解率为76.87%。

Lactose hydrolysis rate was used as evaluation index to study the hydrolysis efifect of lactose in milk powder catalyzed by acidic lactase. The effects of E/S, temperature, pH and time on the hydrolysis rate of lactose in milk powder were investigated. The hydrolysis process conditions were initially determined by single factor experiments. On the basis of single factor experiment, the response surface model analysis of 3 factors and 3 levels was used to determine the optimal technology conditions for enzymatic hydrolysis of lactose in milk powder: E/S 0.16%, 45.31 Ti, pH 4.95. Under these conditions of process variables, the hydrolysis of lactose in milk powder was 76.87%.