菊花CmTCP7基因的克隆及表达分析

Cloning and Expression Analysis of CmTCP7 in Chrysanthemum morifolium

前期转录分析发现TCP7-like基因在菊花舌状花中较特异地高表达,为了明确菊花头状花序形态建成中舌状花的有无及其分子调控机制,该研究以菊花‘粉地毯’(Chrysanthemum morifolium ‘Fen Ditan’)为材料,用RT-PCR和RACE的方法克隆了TCP7-like基因,命名为CmTCP7(登录号:MK140598)。CmTCP7开放阅读框全长为786 bp,编码261个氨基酸。CmTCP7蛋白具有典型的螺旋-环-螺旋的TCP结构域,属植物特有的TCP转录因子家族Ⅰ类成员,为亲水性不稳定蛋白,主要定位于细胞核中。实时荧光定量PCR结果显示,CmTCP7在菊花小花花芽分化时期表达量较高,可能参与小花花芽的形成;而在头状花序开放时期,CmTCP7在舌状花尤其是舌状花花瓣中高表达,推测其能够促进舌状花花瓣的生长,参与形成两侧对称的舌状花。

Previous transcriptome analysis has revealed that a TCP7-like gene is highly expressed in the ray florets of Chrysanthemum morifolium. In order to clarify the presence or absence of ray florets and its molecular regulation mechanism in the formation of chrysanthemum capitulum, we cloned the TCP7-like gene from C. morifolium ‘Fen Ditan’ by RT-PCR and RACE, and named CmTCP7(accession number: MK140598). The open reading frame(ORF) of CmTCP7 was 786 bp, encoding a protein consisting of 261 amino acids with a typical helix-loop-helix TCP domain. CmTCP7 was a hydrophilic unstable protein which belongs to class Ⅰ of plant-specific TCP family and primarily localized to the cell nucleus. The results of quantitative real-time PCR showed that CmTCP7 was highly expressed during floral bud differentiation of chrysanthemum, suggesting that CmTCP7 may be involved in the formation of floral buds. During the flowering time, CmTCP7 was found to be highly expressed in ray florets, especially in ray petals, indicating that CmTCP7 may promote the growth of ray petals and participate in the formation of zygomorphic ray florets.