类风湿性关节炎患者外周血IL-31水平及其与抗环瓜氨酸肽抗体的相关性

IL-31 levels in peripheral blood of patients with rheumatoid arthritis and its correlation with anti-cyclic citrullinated peptide antibody

目的检测类风湿性关节炎(RA)患者外周血中白细胞介素31 (IL-31)、抗环瓜氨酸肽抗体(ACPA)水平,探讨IL-31与ACPA的相关性。方法选取2017年1月至2018年1月在遵义医科大学附属医院确诊的57例RA患者作为RA组,同时将研究期间于我院体检的健康体检者28例作为对照组,采集两组受检者的外周静脉血,分离血清,采用酶联免疫吸附法(ELISA)检测外周血IL-31水平,电化学发光法检测ACPA水平,并应用采用Pearson相关分析法分析IL-31与ACPA的相关性。结果 RA组患者外周血IL-31和ACPA水平分别为(1 367.28±2 129.15) pg/mL和(323.00±379.21) U/mL,均明显高于对照组的(226.66±432.95) pg/mL和(11.28±5.16) U/mL,差异均有统计学意义(P<0.05);IL-31与ACPA呈显著正相关(r=0.641,P<0.05)。结论 RA患者外周血IL-31水平增高,且与ACPA呈正相关,表明IL-31可能参与RA的发生发展,并起着重要作用。

Objective To detect the levels of interleukin 31 (IL-31) and anti-cyclic citrullinated peptide antibody (ACPA) in peripheral blood of patients with rheumatoid arthritis (RA), and to explore the correlation between IL-31 and ACPA. Methods From January 2017 to January 2018, 57 RA patients diagnosed in the Affiliated Hospital of Zunyi Medical University were enrolled in RA group, and 28 healthy people who underwent physical examination in our hospital during the study period were selected as a control group. Peripheral venous blood of the two groups was collected and serum was separated. IL-31 levels in peripheral blood were detected by enzyme-linked immunosorbent assay (ELISA), and the ACPA levels was detected by electrochemiluminescence. The correlation between IL-31 and ACPA was analyzed by Pearson correlation analysis. Results The levels of IL-31 and ACPA in the peripheral blood of the RA group were (1 367.28±2 129.15) pg/mL and (323.00±379.21) U/mL, respectively, which were significantly higher than corresponding (226.66±432.95) pg/mL and (11.28±5.16) U/mL of the control group, and the differences were statistically significant (P<0.05). IL-31 was significantly positively correlated with ACPA (r=0.641, P<0.05). Conclusion The level of IL-31 in peripheral blood of patients with RA is increased and positively correlated with ACPA, indicating that IL-31 may be involved in the development of RA and plays an important role.