螯合剂辅助乙醇提取猕猴桃皮黄酮的工艺优化

Optimization on the Extraction Process of Flavonoids from Kiwi Fruit Peels

采用螯合剂辅助乙醇浸提法提取猕猴桃果皮中的黄酮,以黄酮得率为考察指标,采用单因素和正交试验探讨乙醇浓度、料液比、提取时间、提取温度、螯合剂(-SH)六偏磷酸钠添加量对黄酮得率的影响。试验结果表明,最佳提取工艺为:乙醇浓度60%,料液比1∶20(g/mL),提取时间70min,提取温度80℃,螯合剂(-SH)六偏磷酸钠添加量0.6%,在此条件下猕猴桃皮黄酮的得率为1.518%。

Ethanol extraction was used associated with a chelator to extract flavonoids from kiwifruit peels,taking the yield of flavonoids as review indicator. The factors of flavonoids extraction including ethanol concentration,solid-liquid ratio,extraction time and temprature,the addition of sodium hexametaphosphate(-SH)were studied by single factor tests and orthogonal tests. The results showed that the optimum condition was that the ethanol concentration of 60 %,solid-liquid radio of 1 ∶ 20(g/mL),extracting time of 70 min,extracting temperature at 80 ℃,the addition of sodium hexametaphosphate(-SH) of 0.6 %.Under these conditions,the yield of flavonoids was 1.518 %.