摘要
通过调查问卷方式得知2010-2016年玉林市犬只饲养量年均20.13万头,免疫犬年均为13.64万头,免疫密度年均为67.74%,仍存在未经免疫的犬。ELISA方法检测免疫犬抗体阳性率为91.57%(1509/1648),非免疫犬为4.13%(10/242);RT-PCR方法检测520份犬脑组织,病原检出率为5.00%(26/520)。通过数据分析与实际情况结合,表明玉林市犬狂犬病免疫抗体合格率较高,疫苗效果表现稳定,外观健康犬仍携带狂犬病病毒。
According to our questionnaire,201 300 dogs were raised yearly during 2010 to 2016 in Yulin City,but only 136400 of the dogs were vaccinated,accounting for 67.74% of the total,and those unvaccinated dogs pose dangers to the local people.We used the enzyme linked immunosorbent assay(ELISA) to examine the dogs and found the positive rates of the antibody in the vaccinated and unvaccinated dogs were 91.57%(1509/1648) and 4.13%(10/242),respectively;and the reverse transcription-polymerase chain reaction(RT-PCR) was sued to find that the pathogen detection rate was 5.00%(26/520) from the brain of 520 dogs Combined with the actual situation,the present results showed that the qualified rate of the rabies immune antibody was quite high,and the effect of the vaccine was stable,but the apparently healthy dogs still carry the rabies virus in Yulin Ctiy.
作者
甘一波
段振华
黄宇
张红云
刘香林
黄荣春
GAN Yibo;DUAN Zhenhua;HUANG Yu;ZHANG Hongyun;LIU Xianglin;HUANG Rongchun(Yulin Animal Disease Prevention and Control Center,Yulin 537000,China;Guangxi Yulin Agricultural School,Yulin 537000,China)
出处
《畜牧与兽医》
北大核心
2019年第5期131-134,共4页
Animal Husbandry & Veterinary Medicine
关键词
狂犬病
流行病学调查
血清学抗体检测
分子生物学病原学检测
rabies
epidemiological investigation
serological antibody detection
molecular biological pathogen detection