PGAM1在涎腺腺样囊性癌中的表达及其临床和生物学意义

Expression of PGAM1 in salivary adenoid cystic carcinoma and its clinical and biological significances

目的:研究磷酸甘油酸变位酶1(phosphoglycerate mutase1,PGAM1)在人涎腺腺样囊性癌(salivary adenoid cystic car-cinoma,SACC)的表达及其临床和生物学意义。方法:收集31例SACC和25例正常腺体组织病理切片,免疫组化法检测两者中PGAM1蛋白的表达水平,分析其表达与临床病理之间的相关性。以人SACC高转移潜能细胞系(SACC-LM)为研究对象,构建PGAM1-siRNA干扰片段,检测分析PGAM1干扰前后细胞周期、增殖能力、迁移能力和侵袭能力变化;蛋白质免疫印迹法(West-ern blot)检测干扰前后基质金属蛋白酶2(MMP-2)、基质金属蛋白酶9(MMP-9)、神经型钙黏蛋白(N-cadherin)、上皮型钙黏附蛋白(E-cadherin)、波形蛋白(vimentin)表达的变化。结果:PGAM1在31例SACC中20例(64.5%)为高表达,11例(35.5%)为低表达;在25例正常腺体中均为低表达。PGAM1在实体型SACC的表达明显高于筛孔型和腺管型SACC(P<0.05),PGAM1的表达与性别、年龄和发生部位等无关(P>0.05)。PGAM1基因表达下调后细胞的迁移和侵袭能力明显减弱(P<0.05),MMP-2、MMP-9的表达也明显下调(P<0.05),但细胞增殖和细胞周期比例没有变化(P>0.05)。结论:PGAM1在SACC中的表达与其病理分型有关。PGAM1的表达下调可以显著抑制SACC的迁移和侵袭能力。

Objective:To study the expression of phosphoglycerate mutase1(PGAM1)in salivary adenoid cystic carcinoma(SACC and its clinical and biological significance. Methods:PGAM1 expression was detected by immunohistochemistry in 31 cases of SACC and 25 normal glands,and the correlation with the clinical pathology parameter was analyzed. The highly metastatic human salivary adenoid cystic carcinoma cell line(SACC-LM)was transient transfected by PGAM1 siRNA interference clips. Cell cycle changes,cell proliferation,migration,invasion were evaluated. The changes of matrix metalloproteinase(MMP)-2,MMP-9,N-cadherin,E-cadherin and vimentin expression were examined before and after the interference by Western blot. Results:PGAM1 was expressed at a low level in 25 normal glands. In 31 cases of SACC,20 cases(64.5%)expressed PGAM1 at a high level,while 11 cases(35.5%)expressed at a low level. The PGAM1 expression was significantly higher in the solid pattern than that in the tubular and cribriform pattern(P<0.05). The expression of PGAM1 had no correlation with gender,age or tumor location(P>0.05). The down-regulation of PGAM1 had no effect on the proliferation capability and cell cycle of SACC-LM cells(P>0.05),while cells migration and cell invasion were inhibited significantly after PGAM1 was down-regulated(P<0.05). The expression of MMP-2 and MMP-9 was also down-regulated after PGAM1 was inhibited(P<0.05). Conclusion:The expression of PGAM1 was associated with pathological types of SACC;down-regulation of PGAM1 can significantly inhibit the migration and invasion of adenoid cystic carcinoma.