分散液—液微萃取—液相色谱法测定水中常见抗生素的研究

Determination of Common Antibiotics in Water by Dispersive Liquid-Liquid Microextraction Coupled with Liquid Chromatography

目的建立分散液—液微萃取—液相色谱法测定水中9种抗生素。方法水样经萃取浓缩后液相色谱法测定。结果方法在(5.00～50.0)μg/L范围的相关系数为0.998 1～0.999 4;方法的检出限(0.43～1.57)μg/L、定量限(1.33～5.33)μg/L;回收率66.4%～94.0%,相对标准偏差为1.5%～6.5%。结论该方法操作简便、准确,适用于水样中痕量抗生素的测定。

Objectives A method was established for the detection of nine antibiotics in water samples by dispersive liquid-liquid microextraction coupled with liquid chromatography(DLLME-LC). Methods After concentration, extracts were analyzed by LC. Results The calibration curves ranged from 5.00 μg/L to 50.0 μg/L with the correlation coefficients of 0.998 1~0.999 4 for antibiotics. The Limits of Detections(LODs) and limits of quantification(LOQs) were in the range of(0.43~1.57)μg/L and(1.33~5.33)μg/L, respectively. The recoveries for antibiotics were in the range of 66.4%~94.0% with the relative standard deviations(n=5) of 1.5%~6.5%. Conclusions The method was simple, sensitive and accurate, which could be applied to the detection of trace antibiotics in water samples.