摘要
目的建立分散液—液微萃取—液相色谱法测定水中9种抗生素。方法水样经萃取浓缩后液相色谱法测定。结果方法在(5.00~50.0)μg/L范围的相关系数为0.998 1~0.999 4;方法的检出限(0.43~1.57)μg/L、定量限(1.33~5.33)μg/L;回收率66.4%~94.0%,相对标准偏差为1.5%~6.5%。结论该方法操作简便、准确,适用于水样中痕量抗生素的测定。
Objectives A method was established for the detection of nine antibiotics in water samples by dispersive liquid-liquid microextraction coupled with liquid chromatography(DLLME-LC). Methods After concentration, extracts were analyzed by LC. Results The calibration curves ranged from 5.00 μg/L to 50.0 μg/L with the correlation coefficients of 0.998 1~0.999 4 for antibiotics. The Limits of Detections(LODs) and limits of quantification(LOQs) were in the range of(0.43~1.57)μg/L and(1.33~5.33)μg/L, respectively. The recoveries for antibiotics were in the range of 66.4%~94.0% with the relative standard deviations(n=5) of 1.5%~6.5%. Conclusions The method was simple, sensitive and accurate, which could be applied to the detection of trace antibiotics in water samples.
作者
陈文胜
谭慧嘉
罗志彬
钟志雄
胡洁泉
苏广宁
彭婕
CHEN Wensheng;TAN Huijia;LUO Zhibin;ZHONG Zhixiong;HU Jiequan;SU Guangning;PENG Jie
出处
《环境卫生学杂志》
2019年第2期167-171,共5页
JOURNAL OF ENVIRONMENTAL HYGIENE
基金
广东省医学科学技术研究基金<饮用水中多种抗生素快速检测方法和检测质量控制的研究>(20171020161049314)
关键词
分散液-液微萃取
液相色谱法
水
抗生素
dispersive liquid-liquid microextraction
liquid chromatography
water
antibiotics