单分子/单颗粒光学显微成像研究

Recent advances of single molecule/particle imaging with optical microscopic methods

作为一种新型的光学显微成像手段,单分子、单颗粒光学显微成像技术具有高分辨率、高通量、高灵敏度、非接触、无损伤等优点,在各个学科领域得到了广泛应用.结合本课题组的研究方向,本文综述了单分子、单颗粒光学显微成像研究中的单分子检测,单颗粒光学显微成像追踪,超分辨成像以及单颗粒催化四个方面的重要研究进展,并展望了该成像技术在生物医学应用中的发展前景与挑战.

Single-molecule and-particle imaging with optical microscopic methods have been widely used in various fields with the advantages of high resolution, high throughput and excellent sensitivity. This review summarized recent progresses of optical microscopic imaging of single-molecule and-particle in the areas of molecular detection, superresolution imaging and single-particle catalysis. At last, we outlined the challenges and prospect of optical microscopic imaging techniques for single-molecule and-particle assay in biomedical applications.