柱前衍生高效液相色谱法测定聚山梨酯80中过氧化氢的含量

Determination of Hydrogen Peroxide in Polysorbate 80 by HPLC with Precolumn Derivatization Method

基于三苯基膦与过氧化氢可定量反应生成氧化三苯基膦的原理,建立了柱前衍生高效液相色谱法测定聚山梨酯80中过氧化氢含量的方法.采用ZORBAX SB-C18 色谱柱,以乙腈∶水(60∶40)为流动相,流速为1.0ml/min,检测波长为225nm,柱温为30℃.进样量为10μl.结果表明,过氧化氢在0.18~18.00μg/ml 范围内线性关系良好(r=0.9999),平均回收率为95.1%,RSD为2.72%.该方法准确可靠、灵敏度高,可作为聚山梨酯80 中过氧化氢的含量测定方法.

An HPLC method with precolumn derivatization for determination of hydrogen peroxide in polysorbate 80 was established on the basis of the quantitative reaction between triphenylphosphine and hydrogen peroxide to form triphenylphosphine oxide. A ZORBAX SB-C18 column was adopted, with the mobile phase of acetonitrile∶water (60∶40) at the flow rate of 1.0 ml/min. The detection wavelength was 225 nm and the column temperature was 30 ℃. The linear range for hydrogen peroxide was 0.18-18.00 μg/ml(r=0.999 9). The average recovery was 95.1%, with RSDs of 2.72%. This method is accurate, simple and can be used to determine the content of hydrogen peroxide in polysorbate 80.