应用MALDI-TOF-MS自建鲍氏志贺菌蛋白指纹图谱数据库的研究

Application of MALDI-TOF-MS to the study of the self-built datebase of Shigella boydii

目的运用MALDI-TOF-MS技术建立鲍氏志贺菌的蛋白指纹图谱并将其添加至数据库,实现对临床细菌的快速鉴定,便于临床医生对疾病进行快速而有效的控制。方法采用甲酸萃取法进行鲍氏志贺菌蛋白指纹图谱的添加,研究不同的标本前处理方法对鉴定结果的影响,并用新分离的鲍氏志贺菌和大肠埃希菌对图谱进行验证。结果采用新的图谱鲍氏志贺菌提取法鉴定准确率为95%,直接涂抹法准确率为79%,对大肠埃希菌的鉴定准确率为97%。鲍氏志贺菌不同处理方法的鉴定准确率比较,差异有统计学意义(P<(0.05)。结论新添加的鲍氏志贺菌蛋白指纹图谱能快速准确地鉴定鲍氏志贺菌,此图谱可以应用于鲍氏志贺菌的临床快速诊断。

Objective To use MALDI-TOF-MS to establish Shigella boydii protein fingerprint and add it the database,achieve rapid identification of bacteria for clinicians to control diseases quickly and effectively. Methods Extraction method was used to create the database of mass spectrum map of Shigella boydii,and study the effects of pretreatment methods on identification results,and verified the protein fingerprint by using newly isolated Shigella boydii and Escherichia coil. Results In the new protein fingerprint,the identification rate of Shigella boydii was 95%,but identification rate was 79% by acid extraction,the identification rate of Escherichia coil was 97%.There was statistical significance in different identification methods of Shigella baumannii ( P <0.05). Conclusion The newly added Shigella boydii protein fingerprint can rapidly and accurately identify Shigella boydii,and this fingerprint can be applied to the rapid clinical diagnosis of Shigella boydii.