不同采收期灯盏细辛中灯盏花乙素含量动态积累研究

Study on Dynamic Accumulation of Scutellarin in Differernt Harvesting Periods of Erigeron breviscapus

[目的]研究灯盏细辛中有效成分灯盏花乙素的积累动态。[方法]采用反相高效液相色谱法(RP-HPLC)测定灯盏细辛中灯盏花乙素的含量,色谱柱:菲罗门ODS(150mm×4.6mm,4μm),流动相:甲醇-0.1%磷酸水溶液(40:60),检测波长335nm。[结果]样品平均加样回收率为95.68%,RSD为1.55%(n=6)。灯盏细辛药材中灯盏花乙素的含量在不同采收期有明显的变化规律,而同一采收期不同的药用部位则无明显差异。[结论]该研究为灯盏细辛的规范化种植研究提供科学依据。

[Objective] The research aimed to study the dynamic accumulation of scutellarin in differernt harvest periods of Erigeron breviscapus.[Method]RP-HPLC was used to measure the content of scutellarin,chromatographic column ODS(150 mm×4.6 mm,4 μm),mobile phase methanol-0.1% phosphoric acid solution(40∶60),the detection wave-length was 355 nm.[Result]The average recoveries were 95.68%,RSD was 1.55%.The content of scutellarin in Erigeron breviscapus varied obviously in different harvesting periods,but there was no significant difference in different parts during the same harvesting period.[Conclusion]This study provides a scientific basis for the standardized planting research of Erigeron breviscapus.