甘蔗响应梢腐病菌侵染的蛋白质组学分析

Proteomic Analysis of Different Sugarcane Genotypes in Response to Pokkah Boeng Disease

本文旨在解析甘蔗响应梢腐病菌侵染过程中的生理生化机制,为甘蔗抗梢腐病育种及病害防治提供理论指导和科学参考。以我国甘蔗梢腐病主要致病菌Fusarium verticillioides孢子悬浮液为病原,以高抗梢腐病品种YT94/128和高感梢腐病品种GT37为宿主材料,在温室条件下进行针刺法接种,提取病情指数最严重时期,即接种后第14天的甘蔗叶片蛋白质进行iTRAQ定量表达分析。结果显示,从GT37中成功鉴定3707个蛋白,获得542个差异表达蛋白,其中上调表达187个,下调表达355个;从YT94/128中成功鉴定到3068个蛋白,获得差异蛋白449个,其中上调表达191个,下调表达258个。这说明遗传背景不同的甘蔗品种在蛋白质组成上有很大差异,推测这是不同甘蔗品种间抗梢腐病性差异的重要分子基础。

This study was to provide a theoretical guidance and scientific reference for sugarcane resistance breeding and disease prevention by analyzing the physiological and biochemical mechanism in the infection process of sugarcane response to pokkah boeng disease. With spore suspension of Fusarium verticillioides(the mainly pathogenic fungus of pokkah boeng disease in China) as the pathogeny and YT94/128(HR), GT37(HS) as the tested materials by syringe inoculation under a greenhouse, and the samples were collected at the 14 th post-inoculation with the highest disease index to carry out quantitative expression analysis by isobaric tags for relative and absolute quantitation(iTRAQ). The results showed a total of 3707 proteins were successfully identified from GT37, and 542 differentially expressed proteins(DEPs) were obtained, of which 187 were up-regulated and 355 were down-regulated. Meanwhile, a total of 3068 proteins were successfully identified from YT94/128, and 449 DEPs were obtained, of which 191 were up-regulated and258 were down-regulated. In a word, there are significant differences in the protein composition of sugarcane varieties with different genetic backgrounds, and it is speculated that this is the important molecular basis for the difference of resistance on pokkah boeng disease between different sugarcane varieties.