响应面法优化超声波提取槟榔多糖工艺及其抗炎活性

Optimization of Ultrasound-assisted Extraction of Areca Nut Polysaccharides based on Response Surface Methodology and Anti-inflammatory Activity

采用响应面组合设计优化超声波提取未成熟的槟榔籽多糖的工艺。结果表明,提取功率、提取液料比和提取时间均能显著影响槟榔多糖的得率,且在预测的最佳工艺参数(提取功率630 W,提取液料比20.76mL/g,提取时间501s)条件下,槟榔多糖的实际提取率为(3.3206±0.0099)%。另外,槟榔多糖可以抑制脂多糖(LPS)诱导的巨噬细胞(RAW 264.7)内一氧化氮的生成,其IC50为85.64μg/mL,说明槟榔多糖具有一定的抗炎潜力。

This study determined the optimal conditions for ultrasound-assisted extraction of a polysaccharide,Areca nut polysaccharide,from the seed of Areca nut using response surface methodology.The optimal extraction ultrasonic power,ratio of liquid to raw material,and extraction time were 630W,20.76 mL/g and 503s,respectively,giving a yield of(3.3206±0.0099)%.The result showed that Areca nut polysaccharide inhibited NO production in lipopolysaccharide(LPS)treated RAW 264.7 cells with a dose-dependent manner.The IC50 value was 85.64 μg/mL, which indicated that areca nut polysaccharide had anti-inflammatory potential.