PKG、PKA及VASP在人结直肠癌组织中的表达及临床意义

Expressions and clinical significances of PKG,PKA and VASP in human colorectal cancer

目的探讨cGMP依赖性蛋白激酶(PKG)两种亚型PKG-Ⅰ和PKG-Ⅱ、cAMP依赖性蛋白激酶A(PKA)、血管扩张刺激磷蛋白(VASP)及其两种磷酸化蛋白p-VASP Ser157和p-VASP Ser239在结直肠癌(CRC)组织中的表达水平及临床意义。方法收集云南省肿瘤医院结直肠外科2015年3-9月收治的20例有完整病理资料的CRC患者通过手术切除获得的癌组织及其同源的正常癌旁组织,用Western blot检测上述组织中的PKG-Ⅰ、PKG-Ⅱ、PKA、VASP、p-VASP Ser157和p-VASP Ser239的蛋白表达水平,进行统计分析。同时,分析这些蛋白表达与年龄、性别等病理数据的相关性。结果 CRC组织中的PKG-Ⅰ、PKG-Ⅱ、VASP、p-VASP Ser157和p-VASP Ser239的蛋白表达均显著低于正常的癌旁组织(P<0.05),而PKA差异无统计学意义(P>0.05);并且所有蛋白因子和性别、年龄、肿瘤分化程度及TNM分期等一般病理数据无显著性相关。结论 PKG两种亚型与VASP及其两种磷酸化蛋白在CRC组织显著降低,可能会成为临床诊断治疗CRC的新靶点。

Objective To investigate the expressions and clinical significances of cGMP-dependent protein kinase(PKG)type I(PKG-Ⅰ)and type II(PKG-Ⅱ),cAMP-dependent protein kinase(PKA),vasodilator-stimulated phosphoprotein(VASP)and phosphorylation of VASP including p-VASP Ser157 and p-VASP Ser239 in colorectal cancer(CRC).Methods CRC tissues and their homologous normal paracancerous tissues surgically of 20 patients with complete pathological data from March 2015 to September 2015 admitted to the colorectal surgery department of Yunnan Cancer Hospital were collected.The protein expressions of PKG-Ⅰ,PKG-Ⅱ,VASP,p-VASP Ser157,and p-VASP Ser239 in the above tissues were performed by Western blot.The correlation between these protein expression and general pathology data were analyzed.Results In CRC tissues,protein expressions of PKG-Ⅰ,PKG-Ⅱ,VASP,p-VASP Ser157 and p-VASP Ser239 were significantly lower than those in the normal tissues(P<0.05)while PKA difference was not statistically significant(P>0.05).In addition,there was no significant correlation between all protein factors and general pathological data such as gender,age,tumor differentiation degree and TNM stage.Conclusion Two subtypes of PKG,VASP and its two phosphorylated proteins significantly reduced in CRC tissues,which may be potential targets for CRC therapy.