COL4A1在肝癌中的表达及其对肝癌细胞迁移、侵袭能力的影响

The expression of COL4A1 and its effect on migration and invasion in hepatocellular carcinoma

目的探讨血清Ⅳ型胶原蛋白基因α1(COL4A1)在肝癌组织中的表达,分析敲低COL4A1表达对肝癌细胞迁移及侵袭能力的影响。方法采用Western blot法检测COL4A1在68例肝癌及配对癌旁组织的表达水平;运用siRNA技术敲低肝癌细胞COL4A1表达,采用CCK-8试验检测其对肝癌细胞增殖能力的影响,采用Transwell试验检测其对肝癌细胞迁移和侵袭能力的影响。结果生物信息学分析提示COL4A1mRNA在肝癌中高表达(P<0.01);COL4A1在肝癌组织表达水平明显高于癌旁组织(P<0.05),敲低COL4A1表达明显降低肝癌细胞的增殖、迁移和侵袭能力(P<0.05)。结论 COL4A1在肝癌组织中高表达,并促进肝癌细胞的增殖、迁移和侵袭。

Objective To explore the expression of collagen typeⅣalpha 1(COL4 A1)in hepatocellular carcinoma(HCC)and its effects on migration and invasion of HCC cells.Methods The expression of COL4 A1 68 cases of HCC and its matched adjacent tissue were detected by Western blot.The effect of COL4 A1 knockdown by siRNA on proliferation,migration and invasion was evaluated by CCK-8 assay and Transwell.Results The mRNA expression of COL4 A1 is significantly upregulated in HCC tissues for the first time(P<0.01).As compared to the adjacent tissues,COL4 A1 significantly overexpressed in HCC tissues(P<0.05).Downregulation of COL4 A1 could inhibit the proliferation,migration and invasion ability of HCC cells(P<0.05).Conclusion COL4 A1 is significantly upregulated in HCC and contributes to the proliferation,migration and invasion of HCC cells.