清肺消炎丸对不可分型流感嗜血杆菌诱导肺部炎症小鼠的影响

Effects of Qingfei Xiaoyan Pills on lung inflammation mice induced by non-typeable Haemophilus influenzae

目的探讨清肺消炎丸对不可分型流感嗜血杆菌诱导肺部炎症小鼠的影响。方法 50只小鼠随机分为空白组、模型组、氨苄西林组(0.288 mg/kg)、麻杏石甘汤组(6.05 g/kg)、清肺消炎丸组(2.16 g/kg),每组10只。给药7 d后,测定一般情况(体质量及肺、脾、胸腺指数),HE染色观察肺组织病理变化,ELISA法检测血清炎性细胞因子(IL-1β、IL-6、GCSF、TNF-α)水平,Western blot及RT-PCR法分别检测肺组织TLR4、MyD88、TRAF6蛋白及mRNA表达。结果与模型组比较,清肺消炎丸组一般情况显著改善(P<0.01),炎性细胞因子水平,TLR4、MyD88、TRAF6蛋白及mRNA表达显著降低(P<0.01)。结论清肺消炎丸可缓解不可分型流感嗜血杆菌诱导肺部炎症小鼠肺损伤和炎症,其机制可能与调控被激活的TLR4-MYD88-TRAF6信号通路、抑制炎性细胞因子分泌有关。

AIM To explore the effects of Qingfei Xiaoyan Pills on lung inflammation mice induced by nontypeable Haemophilus influenzae.METHODS Fifty mice were randomly divided into blank group,model group,ampicillin group(0.288 mg/kg),Maxing Shigan Decoction group(6.05 g/kg)and Qingfei Xiaoyan Pills group(2.16 g/kg),10 mice in each group.After 7-day administration,general conditions(body weight and lung,spleen,thymus indices)were determined,pathological changes in lung tissue were detected by HE staining,the levels of serum inflammatory cytokines(IL-1β,IL-6,GCSF,TNF-α)were detected by ELISA,and TLR4,MyD88,TRAF6 protein and mRNA expressions in lung tissue were detected by Western blot and RT-PCR,respectively.RESULTS Compared with the model group,the Qingfei Xiaoyan Pills group demonstrated significant improvement in general conditions(P<0.01),and marked decrease in inflammatory cytokine levels,TLR4,MyD88,TRAF6 protein and mRNA expressions(P<0.01).CONCLUSION Qingfei Xiaoyan Pills can alleviate lung injury and inflammation in lung inflammation mice induced by non-typeable Haemophilus influenzae,whose mechanisms may contribute to the regulation of TLR4-MYD88-TRAF6 signaling pathway and inhibition of inflammatory cytokine secretion.