Myc可控表达的B细胞淋巴瘤小鼠模型的建立

Establishment of a Myc-inducible mouse model of B-cell lymphoma

目的 建立Myc可控表达的B细胞淋巴瘤小鼠模型。方法 将p53敲除小鼠的骨髓细胞与编码Myc雌激素受体融合蛋白(MycER)病毒包装的细胞混合后注入小鼠皮下。腹腔注射他莫昔芬(TAM),1次/d。结果 正常对照组小鼠未出现肿瘤;模型组小鼠持续注射TAM后,肿瘤迅速生长。停止注射TAM后,肿瘤大小迅速减小。3周后,肿瘤恢复生长,但速度较慢;此时若注射TAM,肿瘤生长异常加速。结论 本研究通过TAM实现对Myc“失活”及“激活”两种状态的调控,成功建立Myc可控表达的B细胞淋巴瘤小鼠模型。

Objective To establish a Myc-inducible mouse model of B-cell lymphoma. Methods Mixture of fresh p53-null bone marrow cells and virus-packaging cells producing Myc estrogen receptor (MycER) retrovirus were injected into mice subcutaneously. Tamoxifen (TAM) was injected intra-peritoneally once a day. Results B lymphomas developed and were growing fast with injection of TAM, while there was no tumor development without injection of TAM. However, tumor growth was dramatically repressed after withdrawal of TAM. Interestingly, tumors grew slowly again three weeks later even without TAM injection, and abnormal acceleration of tumor growth occurred if TAM was re-administrated. Conclusion In this study, we successfully establish a B-cell lymphoma mouse model with controllable expression of Myc through TAM to regulate the inactivation and activation of Myc.