微RNA hsa-miR-125a-5p通过激活p53诱导肺癌SPC-A-1细胞凋亡的研究

Effect of microRNA hsa-miR-125a-5p on inducing apoptosis of SPC-A-1 lung cancer cells by activating p53

为了探讨微RNA hsa-miR-125a-5p对肺癌细胞凋亡的影响及其可能的调控机制,通过实时定量RT-PCR (qRT-PCR法)检测hsa-miR-125a-5p在不同细胞系表达的相对含量,流式细胞术检测肺癌细胞的凋亡情况,Western blot和RT-PCR检测p53蛋白和mRNA表达情况,进一步的功能性实验选择SPC-A-1细胞进行。结果显示,与人支气管上皮HBE细胞相比较,hsa-miR-125a-5p在不同的肺癌细胞系中表达下降。在功能获得性实验中,hsa-miR-125a-5p促进凋亡,hsa-miR-125a-5p过表达能够增加野生型p53 mRNA和蛋白表达;阻断野生型p53能够减弱hsa-miR-125a-5p在凋亡中的作用。在功能丧失性实验中,阻断hsa-miR-125a-5p能够降低野生型p53的mRNA和蛋白表达,阻断野生型p53同样能够减弱hsa-miR-125a-5p抑制剂在凋亡中的作用。最后,在p53缺陷细胞系H1299中转染正义或反义miR-125a-5p混合物,结果显示凋亡率均没有明显变化。上述研究表明,微RNA hsa-miR-125a-5p在肺癌细胞SPC-A-1中通过激活p53诱导凋亡。这些结果将为miR-125a家族在肺癌中的作用提供新的视点。

In order to investigate the effect of hsa-mir-125a-5p on apoptosis of lung cancer cells and its possible regulatory mechanism, the relative expression of hsa-mir-125a-5p in different cell lines was detected by real-time quantitative RT-PCR (qRT-PCR), and the apoptosis of lung cancer cells was detected by flow cytometry.The p53 and mRNA expression were tested by Western blot and semi-quantitative RT-5p, respectively.It was found that, compared with human bronchial epithelial (HBE) cells, the expression of hsa-mir-125a-5p decreased in different lung cancer cell lines.In functional acquisition experiments, hsa-miR-125a-5p promoted the apoptosis.Overexpression of hsa-miR-125a-5p could increase wild-type p53 mRNA and protein expression.Further, blocking wild type p53 could attenuate the role of hsa-miR-125a-5p in apoptosis.In the experiment of functional loss, blocking hsa-mir-125a-5p could decrease the expression of wild-type p53 gene and protein.Blocking wild type p53 also attenuated the role of hsa-miR-125a-5p inhibitors in apoptosis.Finally, in p53-deficient cell line H1299, just or antisense miR-125a-5p mixture was transfected.The results showed that there was no significant change in apoptotic rate.It is concluded that mRNA hsa-miR-125a-5p can induce apoptosis of lung cancer cell SPC-A-1 by activating p53.These results will provide a new perspective for the role of miR-125a family in lung cancer.