SCP1对人乳腺癌细胞迁移和增殖的影响及其机制

The Effect of SCP1 and Its Mechanisms on the Migration and Proliferation of Human Breast Cancer Cells

SCP1是一种膜定位的磷酸酶,可以去磷酸化RNA聚合酶Ⅱ并沉默神经元基因.目的:探讨SCP1对人乳腺癌MDA-MB-231细胞迁移和增殖能力的影响,并研究其机制.方法:荧光定量PCR法检测多种癌细胞中SCP1的表达;体外划痕实验检测SCP1对MDA-MB-231细胞迁移能力的影响;Transwell TM细胞侵袭实验检测SCP1对MDA-MB-231细胞侵袭能力的影响;MTT法检测SCP1对MDA-MB-231细胞增殖能力的影响;Western blot法检测SCP1对细胞信号通路分子p-AKT表达的影响.结果:SCP1多种癌细胞中均有表达;SCP1能抑制MDA-MB-231细胞的迁移及增殖能力;抑制SCP1的表达能显著上调MDA-MB-231细胞p-AKT的表达.结论:SCP1可能通过去磷酸化AKT抑制MDA-MB-231细胞迁移和侵袭.

SCP1 as a membrane located phosphatases acts globally to silence neuronal genes and to affect the dephosphorylation of RNA PolⅡ.Objective: To investigate the effect and the relevant molecular mechanisms of SCP1 on the migration and proliferation of human breast cancer MDA-MB-231 cell.Methods: The expression of SCP1 in various cancer cells was measured by qRT-PCR.MTT assay was used to evaluate the proliferation capacity in different MDA-MB-231 breast cancer cells.The effects of SCP1 on the expression of p-AKT in MDA-MB-231 cells were examined by Western blotting.In vitro wound healing assay and Transwell^TM assay were utilized to measure the effects of SCP1 on the migration and invasion capability of MDA-MB-231 cells.Results: Suppressed SCP1 significantly promoted the migration and proliferation of MDA-MB-231 cells.Inhibition of SCP1 did not affect the expression of total AKT,whereas the phosphorylated AKT level was markedly up-regulated.Conclusion: SCP1 could inhibit the migration and proliferation of human breast cancer MDA-MB-231 cell via dephosphorylation of AKT.