建立矮膜苞芹HPLC指纹图谱

Study on HPLC Fingerprint of Hymenoleana nana

【目的】建立矮膜苞芹药材高效液相指纹图谱,为矮膜苞芹药材的质量控制及开发利用提供科学依据。【方法】采用HPLC法,WondaCract ODS-2C18色谱柱(4.6mm×250mm,5μm),流动相乙腈:0.1%甲酸水梯度洗脱,流速1.0mL/min,柱温30℃,检测波长283nm。测定10批药材的指纹图谱,运用《中药色谱指纹图谱相似度评价系统》和统计软件中聚类分析、主成分分析及偏最小二乘法-判别分析对指纹图谱进行分析。【结果】标定出共有色谱峰13个,10批药材中有7批指纹图谱的相似度>0.9。【结论】建立了矮膜苞芹指纹图谱共有模式,所建立的方法具有良好的精密度、稳定性和重复性。

【Objective】To establish the HPLC Fingerprint of Hymenoleana nana and provide a scientific basis for the quality control,the development and utilization of medicinal plant resources.【Method】HPLC was performed using a WondaCract ODS-2 C 18 column(4.6 mm×250 mm,5μm),with of mobile phase acetonitrile:0.1%formic acid water gradient elution,flow rate 1.0 mL/min,column temperature 30℃,detection wavelength 283 nm.The fingerprints of 10 batches of medicinal materials were determined and the fingerprints were analyzed by cluster analysis,principal component analysis and least squares-discriminant analysis using the Similarity Evaluation System for Chromatographic Fingerprint of TCM and statistical software.【Result】The chromatographic evaluation software calibrated 13 common chromatograms,and the similarity of 7 fingerprints in 10 batches was>0.9.【Conclusion】The established method has good precision,stability and repeatability,which lays a foundation for the total quality control of the medicinal materials of Hymenoleana nana.