miR-127在肺癌中的表达及功能

Expression and Function of miR-127 in Lung Cancer

目的探讨miR-127在肺癌中的表达及对其肺癌细胞生物学特性的影响。方法收集20例患者的肺癌组织及癌旁组织,通过实时荧光定量PCR检测miR-127在肺癌及癌旁组织中的表达水平。应用mimics转染A549细胞株,采用平板克隆、MTT法检测miR-127 mimics组、空白载体转染组(NC)肺癌细胞的增殖活性。Transwell实验计算肺癌细胞过膜数量,研究miR-127与肺癌细胞迁移作用的关系。结果 miR-127在肺癌组织中的表达显著低于癌旁正常组织(0.55±0.05 vs. 1.45±0.13, P=0.001)。肺癌组织中miR-127的表达随着病理分期恶性程度的增高而降低(H=6.528, P=0.034)。平板克隆及MTT实验结果显示miR-127 mimics组的细胞活性及增殖明显受抑制(P=0.0032; P=0.0045),Transwell实验显示miR-127 mimics组的细胞过膜数量(79±18/视野)明显低于NC组(352±21/视野),差异有统计学意义(P=0.002)。结论 miR-127在肺癌组织中低表达,其在抑制肺癌细胞的增殖和转移过程中发挥重要作用,可作为肺癌的潜在临床诊断标志物。

Objective To investigate the expression of miR-127 in lung cancer and its effect on biological characteristics of lung cancer cells. Methods The lung cancer and adjacent tissues of 20 patients were collected, and the expression levels of miR-127 in lung cancer and adjacent tissues were detected by real-time polymerase chain reaction(PCR). A549 cell line were transfected with miR-127 mimics. The proliferation activity of lung cancer cells in miR-127 mimics group and negative control(NC) group were detected by cell colony formation assay and MTT assay. Transwell assay were performed to calculate the number of lung cancer cells passing through the membrane, and to analyze the relationship between miR-127 and the migration of lung cancer cells. Results The expression of miR-127 in lung cancer tissues was significantly lower than that in adjacent normal tissues(0.55±0.05 vs. 1.45±0.13, P=0.001). The expression of miR-127 in lung cancer tissues was decreased with the increased malignancy degree of pathological stage(H=6.528,P=0.034). The cell viability and proliferation of miR-127 mimics group were significantly inhibited(P=0.0032;P=0.0045). The number of lung cancer cells passing through the membrane in miR-127 mimics group was significantly lower than that in the NC group(79±18/field vs. 352±21/field, P=0.002). Conclusion The expression of miR-127 is decreased in lung cancer tissues, and it inhibits the proliferation and migration of lung cancer cells. It can be used as a potential clinical diagnostic marker for lung cancer, providing a new idea for early diagnosis and treatment of lung cancer.