结直肠癌组织中微小RNA-449b的水平及临床意义

Clinical significance and level of microRNA-449b in colorectal cancer tissues

目的探讨微小RNA-449b(miR-449b)在结直肠癌组织中的表达及其临床意义。方法收集本院2012年1月至2014年12月收治的92例手术切除的结直肠癌组织和88例癌旁正常肠黏膜组织存档蜡块,采用实时定量PCR(QPCR)法检测以上组织中miR-449b的表达情况,并分析其与临床病理特征(性别、年龄、部位、浸润深度、淋巴结转移、远处转移、TNM分期、分化程度及血清CEA、CA50和CA199水平)的关系,根据随访资料分析miR-449b表达与预后的关系。结果 QPCR检测发现92例结直肠癌组织中miR-449b水平分别为0.367±0.183,低于癌旁组织的1.029±0.228(P<0.05);miR-449b表达与结直肠癌患者的性别、年龄、部位、分化程度及血清CEA、CA50和CA199水平均无关(P>0.05),而与浸润深度、淋巴结转移、远处转移和TNM分期均有关(P<0.05)。miR-449b低表达者的中位总生存期为37.0(95%CI:33.168～40.382)个月,低于miR-449b高表达者的47.0(95%CI:42.561～51.419)个月(P<0.001)。结论 miR-449b在结直肠癌组织中表达降低,与结直肠癌的发生发展有关,且miR-449b低水平的预后较差,在该类型恶性肿瘤的诊断和预后预测上有一定价值。

Objective To investigate the expression of microRNA-449b (miR-449b) in colorectal cancer and its clinical significance. Methods Ninety-two cases of resected colorectal cancer tissues and 88 cases of paracancerous normal intestinal mucosa tissues were collected from January 2012 to December 2014. The expression of miR-449b in the above tissues were detected by real-time quantitative PCR (QPCR), and their relationship with clinicopathological parameters (sex, age, location, depth of invasion, lymph node metastasis, distant metastasis, TNM stage, differentiation and serum levels of CEA, CA50 and CA199) were analyzed. The relationship between the expression of miR-449b and prognosis was analyzed according to follow-up data. Results The level of miR-449b in 92 colorectal cancer tissues was 0.367±0.183, lower than 1.029±0.228 in adjacent tissues ( P < 0.05). The expression of miR-449b was not correlated with sex, age, location, differentiation degree and serum CEA, CA50 and CA199 levels of colorectal cancer ( P > 0.05), but with invasion depth, lymph node metastasis, distant metastasis and TNM stage ( P < 0.05). Univariate analysis showed that the median overall survival of those with low expression of miR-449b was 37.0 (95%CI: 33.168-40.382) months, lower than 47.0 (95%CI: 42.561-51.419) months in those with high expression of miR-449b ( P < 0.001). Conclusion The expression of miR-449b decreased in colorectal cancer tissues, which is related to the occurrence and development of colorectal cancer, and the prognosis of patients with low levels of these two indicators is poor, which has certain value in the diagnosis and prognosis evaluation of this type of malignant tumors.