摘要
合成了1个新型的双核钌配合物,并通过紫外-可见吸收光谱和荧光光谱滴定、热熔链、盐效应、黏度和凝胶电泳实验研究了其与小牛胸腺DNA (ct-DNA)之间的相互作用.结果表明,由于主配体的位阻作用,该配合物通过部分插入模式与DNA键合,键合常数大于10~6 L/mol;在365 nm紫外光照射下,该配合物能够断裂pBR322 DNA,是有效的DNA断裂试剂.
A dinuclear ruthenium complex has been synthesized and characterized. The calf thymus DNA (ct-DNA) binding properties of the complex are investigated by means of DNA viscosity and optical spectroscopic techniques of UV-visible absorption and emission spectral titrations, DNA thermal denaturation and reverse, salt effect and agarose gel electrophoresis. The results suggest that the complex binds to DNA through partial-intercalation mode with a large DNA binding constant of 10 6 L/mol order of magnitude. The complex is also demonstrated to be an efficient cleaver of pBR 322 DNA under irradiation at 360 nm.
作者
王爽
徐雪雪
米雅萱
郑泽宝
赵晓珑
WANG Shuang;XU Xuexue;MI Yaxuan;ZHENG Zebao;ZHAO Xiaolong(College of Chemistry and Environmental Science,Hebei University,Baoding 071002,China;College of Chemistry, Taishan University, Taian 271021,China)
出处
《河北大学学报（自然科学版）》
CAS
北大核心
2019年第3期254-263,共10页
Journal of Hebei University(Natural Science Edition)
基金
国家自然科学基金资助项目(21401038)
河北省自然科学基金资助项目(B2016201122)
河北省高等学校科学研究计划项目(QN2014123)
保定市科学技术研究与发展计划项目(14ZF009)
泰安市科学技术研究与发展计划项目(2015GX2048)
关键词
钌配合物
DNA
键合
电泳
ruthenium complex
DNA
bind
electrophoresis
