JAK/STAT3信号通路参与白细胞介素-17诱导的支气管平滑肌细胞增殖与迁移被引量：10

JAK/STAT3 Signaling Pathway Is Involved in Promoting the Proliferation and Migration of Bronchial Smooth Muscle Cells Induced by Interleukin-17

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摘要【目的】探讨白介素-17(IL-17)促进支气管平滑肌细胞(BSMC)的增殖与迁移作用及JAK/STAT3相关信号通路在其中的作用机制。【方法】使用不同浓度IL-17处理BSMC不同时间,以确定最佳实验条件。随后使用MTT检测细胞活力,BrdU染色观察细胞增殖状态,并使用PI染色流式细胞仪检测细胞周期。进一步利用细胞迁移实验检测细胞迁移能力。使用Western blot实验检测IL-17处理后BSMC的JAK、p-JAK、STAT3、p-STAT3蛋白表达量。利用JAK/STAT3信号通路特异性阻断剂AG490来特异性阻断JAK/STAT3信号通路,检测阻断JAK/STAT3信号后IL-17对细胞增殖、迁移以及JAK/STAT3信号通路蛋白表达量的影响。【结果】IL-17可促进BSMC增殖(P <0.05),促进细胞周期进程(P <0.05)并使细胞迁移能力增强(P <0.05)。这一过程伴随着BSMC的JAK/STAT3信号通路的增强(P <0.05)。抑制JAK/STAT3信号通路缓解了IL-17对BSMC的促进增殖和迁移的作用(P <0.05)。【结论】JAK/STAT3信号通路参与了IL-17诱导的BSMC增殖与迁移作用,AG490对IL-17引起的BSMC JAK/STAT3信号通路的增强起到了抑制作用。【Objective】To investigate the effects of interleukin-17(IL-17)on the proliferation and migration of bronchial smooth muscle cells(BSMC)and the role of JAK/STAT3 signaling pathway in this process.【Methods】BSMC were treated with different concentrations of IL-17 for different times to determine the best of the experimental condition.Then MTT assay was used to detect cell viability. Cell proliferation states were detected by BrdU staining,and the cell cycle was assessed by PI staining using a flow cytometer. Transwell cell migration assay was further used to detect cell migration ability. The expression of JAK,p-JAK,STAT3 and p-STAT3 in BSMC after being treated with IL-17 was detected by Western blotting. JAK/STAT3 signaling pathway specific blocker AG490 was used to investigate the role of JAK/STAT3 signalingpathwayinIL-17-inducedBSMCproliferationandmigration.TheeffectsofIL-17 oncellproliferation,migration and JAK/STAT3 signaling pathway related protein expression were evaluated after blocking the JAK/STAT3 signaling.【Results】IL-17 enhanced the proliferation(P<0.05),promoted the cell cycle transitions(P<0.05)and significantly increases the migration ability(P<0.05)in BSMC. This process was accompanied by the enhancement of JAK/STAT3 signaling pathway in BSMC(P<0.05). Inhibition of JAK/STAT3 signaling pathway alleviated BSMC proliferation and migration induced by IL-17(P<0.05).【Conclusions】JAK/STAT3 signaling pathway participates in the stimulation process of IL-17 on the proliferation and migration of BSMC. AG490 inhibits the enhancement of JAK/STAT3 signaling pathway in BSMC induced by interleukin-17.

作者蓝引乐朱妤王建华凌元亮 LAN Yin-le;ZHU Yu;WANG Jian-hua;LING Yuan-liang(Department of Respiration,ZhejiangUniversity School of Medicine,Hangzhou 310012,China;Department of Traditional Chinese Medicine,The Children's Hospital,Zhejiang University School of Medicine,Hangzhou 310012,China;Community Health Center,Xiang Fu Street,Gongshu District,Hangzhou 310023,China)

机构地区浙江大学医学院附属儿童医院呼吸科浙江大学医学院附属儿童医院中医科杭州市拱墅区祥符社区卫生服务中心

出处《中山大学学报（医学版）》 CAS CSCD 北大核心 2019年第3期379-386,共8页 Journal of Sun Yat-Sen University:Medical Sciences

基金浙江省卫生计生委项目(2012KYA120)

关键词白介素-17 支气管平滑肌细胞 JAK/STAT3通路细胞增殖细胞迁移 interleukin-17 bronchial smooth muscle cells JAK/STAT3 pathway cell proliferation cell migration

分类号 R363.1 [医药卫生—病理学]

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