清脑益元汤对大鼠脑缺血再灌注损伤TNF-α、IL-8表达的影响

Effect of Qinnao Yiyuan Decoction on TNF-α and IL-8 levels in Modelrats with Cerebra Lischemia Reperfusion Injury

目的:观察清脑益元汤对大鼠脑缺血再灌注损伤肿瘤坏死因子(TNF-α)、白细胞介素(IL-8)表达的影响。方法:将大鼠随机分为空白组,假手术组,脑缺血再灌注组,清脑益元汤组4组。除空白组外,各组均按大鼠脑缺血2 h后,再灌注时间点3、6、12、24、48 h分为5个亚组,每组6只。造模成功后取大鼠缺血侧脑组织,采取免疫组化法检测TNF-α、IL-8的表达,并行HE染色镜下观察大鼠脑组织的病理形态变化。结果:HE染色:(1)空白组、假手术组大鼠脑组织病理形态无明显改变;(2)脑缺血再灌注组大鼠神经元结构明显受损,清脑益元汤组较脑缺血再灌注组在各时间点神经元受损减轻。免疫组化法:(1)大鼠MACO术后,与空白组及假手术组比较,脑缺血再灌注组和清脑益元汤组TNF-α、IL-8阳性细胞的表达在各时间点均升高,差异有统计学意义(P<0.01);(2)脑缺血再灌注组TNF-α、IL-8阳性细胞表达在各时间段均高于清脑益元汤组(P<0.05),两组大鼠在缺血再灌注3 h后,TNF-α、IL-8阳性细胞表达开始增加,6 h显著升高,12 h达高峰,在24 h后呈逐步降低的趋势。结论:清脑益元汤对大鼠脑缺血再灌注后TNF-α、IL-8表达有明显的调控作用,能抑制TNF-α、 IL-8阳性细胞的表达,减轻脑缺血再灌注损伤炎性级联反应,发挥脑保护作用。

Objective: To investigate the effect of QinnaoYiyuan Decoction on the expressions of TNF-α and IL-8 after focal cerebral ischemia reperfusion(I/R) rats. Methods: All SD rats were randomly divided into the normal control group, sham operation group, ischemia-reperfusion group and QinnaoYiyuan Decoction group. Except the normal control group, other groups was further divided into 5 subgroups according to 3 h, 6 h, 12 h, 24 h and 48 h after I/R. The brain was stained by HE and observed with optical microscopes. The changes of TNF-α and IL-8 expressions on the ischemic side of in hippocampus were measured by Immunohisto Chemistry. Results: HE staining:(1)The brain tissue of the normal control group and sham operation group had no significant change in pathological morphology rats.(2)Comparing with ischemia-reperfusion group, the QinnaoYiyuan Decoction group damage of nerve cell was reduced at all time. Immunohistochemistry:(1)Comparing with the normal control group and sham operation group, the ischemia-reperfusion group and QinnaoYiyuan Decoction group, the expressions of TNF-α and IL-8 had increased significantly at all time(P<0.01).(2)As compared with the Qinnao Yiyuan Decoction group, the expressions of TNF-α and IL-8 in the ischemia-reperfusion group had increased significantly at all time(P<0.05). After 3 h of I/R, the expressions of TNF-α and IL-8 in the ischemia-reperfusion group and Qinnao Yiyuan Decoction groups had increased gradually. At 6 h,the expressions were increased significantly and reached the zenith at 12 h and became decreased trend at 24 h. Conclusions: Qinnao Yiyuan Decoction can reduce cerebral ischemia reperfusion injury and play a role of brain protection. It plays a neuron-protective role through reducing the expressions of TNF-α and IL-8.