环氧合酶-2和磷酸化P38丝裂原活化蛋白激酶在扁平苔藓皮损中的表达

Expression of COX-2 and phosphorylated P38 mitogen-activated protein kinase in the lesions of lichen planus

目的:检测环氧合酶-2(COX-2)及其上游调控蛋白磷酸化P38丝裂原活化蛋白激酶(p-P38MAPK)在扁平苔藓皮损中的表达,探讨其在发病中的意义。方法:采用免疫组化技术检测20例扁平苔藓皮损标本中COX-2和p-P38MAPK的表达情况,20例正常皮肤组织作为对照。结果:20例扁平苔藓皮损标本中COX-2和p-P38MAPK的积分光密度值(IOD)分别为(8.46±1.79)×10^3和(11.54±2.85)×10^3,均明显高于对照组(3.14±2.35)×10^3和(6.26±3.12)×10^3,差别具有统计学意义(均P<0.01),同时COX-2与p-P38MAPK的表达强度呈正相关(r=0.62,P<0.01)。结论:扁平苔藓皮损中COX-2表达上调,可能与上游P38MAPK的活化有关,可能是引起扁平苔藓发病的环节之一。

Objective: To detect the expression level of cyclooxygenase-2 (COX-2) and phosphorylated P38 mitogen-activated protein kinase (p-P38MAPK) in the lesions of lichen planus (LP) and to explore its role in the pathogenesis of LP. Methods: The expression level of COX-2 and p-P38MAPK in 20 lesions with LP and normal skin of 20 human controls were detected by immunohistochemical technology. Results: The IOD(integrated optical density) of COX-2 and p-P38MAPK in LP lesions were (8.46±1.79)×10^3 and ( 11.54 ±2.85)×10^3, which were both higher than those in the controls,(3.14±2.35)×10^3 and (6.26±3.12)×10^3, with significant differences ( P s<0.01). There was a positive correlation between the expression level of COX-2 and p-P38MAPK in LP lesions ( r=0.62,P <0.01). Conclusion: The up-regulation of COX-2 expression may be correlated with the activation of upstream P38MAPK, which may be involved in the pathogenesis of lichen planus.