摘要
目的:比较实时荧光定量PCR(qPCR)技术在皮肤结核(CTB)石蜡包埋组织及新鲜组织中的敏感性,以探讨在CTB石蜡包埋组织中开展结核分枝杆菌(MTB)检测的可行性。方法:应用qPCR技术检测临床确诊为CTB的6例新鲜组织样本和20例石蜡包埋组织样本的MTB阳性率。结果:新鲜组织敏感性为50%,石蜡包埋组织敏感性为35%。结论:qPCR技术可检测CTB新鲜组织和石蜡包埋组织中的MTB,前者的敏感性高于后者。
Objective: To compare the sensitivity of Real-time fluorescence quantitative PCR (qPCR) in detecting Mycobacterium tuberculosis (MTB) in cutaneous tuberculosis (CTB) both in paraffin-embedded tissues and fresh tissues,to discuss the feasibility of qPCR detection in paraffin tissues of CTB. Methods: MTB in 6 fresh tissues and 20 paraffin-embedded tissues of CTB were detected by qPCR. Results: The sensitivity rate of fresh tissues and paraffin-embedded tissues were 50% and 35% respectively. Conclusion: QPCR can be applied both in the fresh CTB tissues and paraffin-embedded tissues. The sensitivity of the former is higher than the latter.
作者
满春花
糜自豪
孙乐乐
卢宪梅
王川
王建文
陈学超
刘永霞
陈声利
刘红
张福仁
MAN Chunhua;MI Zihao;SUN Lele;LU Xianmei;WANG Chuan;WANG Jianwen;CHEN Xuechao;LIU Yongxia;CHEN Shengli;LIU Hong;ZHANG Furen(School of Medicine and Life Sciences,University of Jinan-Shandong Academy of Medical Sciences,Jinan 250062,China;Shandong Provincial Institute of Dermatology and Venereology,Jinan 250022,China;Shandong Provincial Hospital for Skin Diseses,Jinan 250022,China;Department of Dermatology Zoucheng People s Hosptal,Zoucheng 273500,China)
出处
《中国麻风皮肤病杂志》
2019年第4期215-218,共4页
China Journal of Leprosy and Skin Diseases
基金
山东省医学科学院医药卫生科技创新工程
关键词
结核
皮肤结核
qPCR
tuberculosis
cutaneous tuberculosis
qPCR
