1.8 GHz射频场辐射对小鼠应激激素和免疫因子的时间毒性

Chronotoxicity of 1.8 GHz radio-frequency radiation on plasma stress hormones and immune factors in mice

目的研究1.8 GHz射频场辐射(radio-frequency radiation, RF)对小鼠应激和免疫功能的昼夜时间毒性。方法将72只具有昼夜节律的雄性C57BL小鼠分为6个RF组和6个假暴露组(Sham)组。6个RF组分别在一天中不同的授时时间(zeitgeber time,ZT;ZT 0:00为早晨7:00),即ZT 0:00、ZT 4:00、ZT 8:00、ZT 12:00、ZT 16:00和ZT 20:00,进行1.8 GHz的射频场辐射(226μW/cm^2),每天2 h,Sham组同样处理,但不给电磁信号。连续60 d后收集血样,用酶联免疫吸附法测定血清中的应激激素促肾上腺皮质激素(ACTH)、皮质醇(CORT)及细胞免疫因子粒细胞-巨噬细胞集落刺激因子(GM-CSF)、肿瘤坏死因子α(TNF-α)。结果 Sham组中ACTH、CORT、GM-CSF、TNF-α日均值分别为84.12、60.14、1112.02和594.49 ng/L,经过连续60 d辐射后分别降至62.07、41.21、84.18和305.08 ng/L(P<0.05)。RF组小鼠血清中CORT、GM-CSF和TNF-α昼夜节律消失(P>0.05),ACTH的振幅从12.45变小到4.88,峰值时间由授时时间1:39推迟至5:29。结论 1.8 GHz射频场辐射可削弱雄性小鼠应激和免疫功能,改变其昼夜节律。

OBJECTIVE To study the chronotoxicity of radio-frequency radiation(RF)on the plasma stress hormones and immune factors in mice.METHODSA total of 72 healthy C57 BL mice with circadian rhythm were divided into twelve groups:6 Sham group and 6 RF groups.RF groups were exposed to 1.8 GHz RF at 226μW/cm^2 for 60 days with 2 h/day respectively at corresponding zeitgeber time(ZT 0:00,ZT 4:00,ZT 8:00,ZT 12:00,ZT 16:00,ZT 20:00).The Sham group mice were exposed to the same condition without electromagnetic signal.At the end of last RF exposure,blood samples were collected from each animal.The concentrations of plasma stress hormones(ACTH,CORT)and immune factors(GM-CSF,TNF-α)were determined by enzyme linked immunosorbent assay(ELISA)method.RESULTS The daily average levels of ACTH,CORT,GM-CSF and TNF-αwere 84.12,60.14,1112.02 and 594.49 ng/L,which were decreased to 62.07,41.21,84.18 and 305.08 ng/L after 60 days of RF exposure.Compared to sham-exposed animals,the daily average levels of ACTH,CORT,GM-CSF and TNF-αwere all significantly decreased(P<0.05).Circadian rhythms in the secreting of CORT,GM-CSF,TNF-αwere disappeared(P>0.05),circadian rhythms of ACTH was shifted in RF-exposed mice,with the amplitude reduced from 12.45 to 4.88 and peak time postponed from 1:39 to 5:29.CONCLUSION 1.8 GHz RF may weaken the function of stress and immune,and disturb their circadian rhythmicities.