摘要
以菊花杂交品种"红粉"×"延红"杂交种子为外植体,研究外植体消毒时间、生长调节剂浓度以及基本培养基浓度对其增殖和生根的影响,旨在建立组培快繁体系。结果表明:0.1%HgCl2消毒8 min,种子发芽率最高为78.3%。在MS+BA 1.0 mg/L+NAA 0.4 mg/L的培养基中,试管苗增殖及生长最佳,株高为9 cm,节数为7.7个。在生根培养中,基本培养基浓度为3/4 MS,当添加IBA 1.0 mg/L时,根生长最佳,根长和根数分别为3.5 cm和15.6条,地上部生长良好,株高和干重均好于其它培养基。将生根的幼苗移栽到栽培基质中(腐殖土∶珍珠岩=3∶1),成活率达95%以上。
To establish a rapid propagation system of ‘Fenghong’בYanhong’ hybrid, seeds of the hybrid as the explant, effects of sterilization time, concentrations of growth regulators and MS medium on shoot proliferation and root growth were determined. The results showed that the maximum seed germination rate reached 78.3% when seeds were sterilized with 0.1% HgCl2 for 8 min. During the proliferation, the optimal culture medium was MS medium supplemented with 1.0 mg/L BA and 0.4 mg/L NAA. Shoot height and number of nodes were 9 cm and 7.7, respectively. During the rooting, root growth was favorable in 3/4 MS medium supplemented with 1 mg/L IBA, where the root length and root numbers per plantlet were 3.5 cm and 15.6, respectively. The growth of the aboveground part, such as shoot height and dry weight was also optimal in 3/4 MS medium+1 mg/L IBA. The survival rate of seedlings was more than 95% when plantlets were transplanted into the cultivation matrix(Humus∶Perlite=3∶1).
作者
岳圆圆
陈慧
全英杰
龚晨
李鑫
高日
YUE Yuanyuan;CHEN Hui;QUAN Yingjie;GONG Chen;LI Xin;GAO Ri(Agricultural College of Yanbian University,Yanji Jilin 133002,China;Jilin Radio and TV University,Changchun Jilin 130000,China)
出处
《延边大学农学学报》
2019年第1期47-50,共4页
Agricultural Science Journal of Yanbian University
基金
吉2018年“十三五”科学技术项目(JJKH20180902KJ)
延大科合字(2017)第21号
延边大学博士科研启动基金(2018)
关键词
试管苗
增殖
生根
菊花
杂交
test-tube culture
proliferation
rooting
Chrysanthemum morifolium
hybridization