丹红注射液对缺血性中风大鼠舌象的干预研究

Intervention of Danhong Injection on Tongue Manifestation in Cerebral Ischemic Stroke Rats

目的观察丹红注射液干预缺血性中风大鼠舌象的作用,为其临床应用提供依据。方法制备缺血性中风大鼠模型,进行神经行为学评分,以丁苯酞注射液为对照,观察丹红注射液对舌象的干预作用。对舌腹面、舌背面拍照记录并分析血瘀证相关舌象。舌色的R、G、B值由Photoshop5.0软件获得,根据标准色卡进行校正计算获得校正R、G、B值。根据舌下脉络的色泽和长短进行评分。进行TTC染色脑梗死体积测算,分析两者相关性。结果模型大鼠舌色校正R、G、B值与脑梗死体积均密切相关(分别为P <0.05,P <0.01,P <0.01)。丁苯酞组、丹红注射液组均能改善模型大鼠舌色,校正R、G、B值显著高于模型组(分别为P <0.01,P <0.05,P <0.05),显著改善舌下脉络色泽,显色长度缩短(均P <0.01);两个治疗组神经行为学评分较模型组显著下降(均P <0.05),梗死灶显著缩小(均P <0.01)。以上指标在丁苯酞及丹红注射液组之间无明显差异(P> 0.05)。结论丁苯酞、丹红注射液均能显著改善缺血性中风大鼠血瘀证相关舌象,促进神经功能恢复,缩小梗死灶。

Objective: To investigate the effect of Danhong Injection on tongue manifestation in cerebral ischemic stroke rats and provide a solid basis for clinical application. Methods: The rat model of ischemic stroke was established and the neurobehavioral score was made. The intervention effect of Danhong Injection on tongue manifestation was observed with butylphthalide injection as control. The ventral and dorsal surface of tongue were photographed and recorded,and the tongue manifestation related to blood stasis syndrome were analyzed. The values of R,G and B of tongue color were obtained with Photoshop software,and corrected by calibration calculation based on standard color cards. The color and length of sublingual veins were evaluated. The volume of cerebral infarction was measured by TTC staining,and the correlation between the two was analyzed. Results: The corrected R,G and B values of tongue color in model rats were closely related to the volume of cerebral infarction(P < 0.05,P < 0.01,P < 0.01,respectively). Butylphthalide and Danhong Injection could improve the tongue color of model rats,and the corrected R,G and B values were significantly higher than those of the model group(P < 0.01,P < 0.05,P < 0.05,respectively),and significantly improved the color of sublingual veins;the chromogenic length was shortened(P < 0.01). The neurobehavioral score was significantly lower than that of the model group (all P < 0.05),and the infarct size was significantly reduced(all P < 0.01) than those in the cerebral ischemic stroke model group. Conclusion: Butylphthalide and Danhong Injection can significantly improve the tongue manifestation related to blood stasis syndrome in ischemic stroke rats,promote the recovery of nerve function and reduce the infarct in cerebral ischemic stroke rats.