香连丸对急性溃疡性结肠炎小鼠氧化应激介导的细胞自噬的影响研究

Effect of Xianglian Pill on Autophagy Mediated by Oxidative Stress in Mice with Acute Ulcerative Colitis

目的观察香连丸对急性溃疡性结肠炎大鼠氧化应激反应及其介导的细胞自噬的影响。方法 60只C57BL/6小鼠随机分为正常组、模型组、香连丸低、中、高剂量组和美沙拉嗪组。除正常对照组外,余下各组大鼠均采用DSS的方法复制UC模型。分别观察各组大鼠结肠组织病理学形态,生化法检测氧化应激指标(MDA、SOD和GSH-px)的含量,Western blotting法检测自噬相关蛋白(Beclin-1、LC3Ⅱ、LC3Ⅰ和p62)的表达。结果香连丸能够显著改善UC小鼠结肠组织形态,降低DAI及组织学损伤评分,与模型组比较差异均具有显著统计学意义(P <0.01);此外,香连丸还能显著下调结肠组织中MDA和LC3Ⅰ的表达,增加SOD及GSH-px的活性、Beclin-1、LC3Ⅱ、p62蛋白的表达及LC3Ⅱ/LC3Ⅰ的比值,与模型组比较差异均具有统计学意义(P <0.05和P <0.01)。结论香连丸可以明显降低氧化应激反应,促进细胞自噬,减轻肠道炎性损伤,促进组织修复。

Objective: To investigate the effect of Xianglian Pill on oxidative stress response and autophagy mediated by oxidative stress response in rats with acute ulcerative colitis. Methods: 60 C57BL/6 mice were randomly divided into the control group,the model group,Xianglian Pill low,medium and high dose groups and mesalazine group. Except the control group,UC model was replicated by DSS in the rest groups. The histopathological morphology of colon was observed;the levels of oxidative stress indexes(MDA,SOD and GSH-px) were detected by biochemical method,and the expressions of autophagy-related proteins(Beclin-1,LC3Ⅱ,LC3Ⅰ and p62) were detected by Weatern blotting. Results: Xianglian Pill significantly improved the colon histomorphology,and reduced DAI and histological damage score of UC mice,with significant differences when compared with the model group(P < 0.01). In addition,Xianglian Pill could significantly down-regulate the expressions of MDA and LC3Ⅰ in colon tissue,and increase the activity of SOD,GSH-px,the expressions of Beclin-1,LC3II and p62 proteins,and LC3Ⅱ/LC3Ⅰ ratio;the differences were significantly when compared with the model group(P < 0.05 and P < 0.01). Conclusion: Xianglian Pill can significantly reduce oxidative stress and promote autophagy,alleviate intestinal inflammatory damage and promote tissue repair.