lncRNA-ADAMTS9-AS2与胶质瘤预后的相关性

Correlativity between low expression level of long non-coding RNA ADAMTS9-AS2 and poor prognosis in patients with gliomas

目的探讨长链非编码RNA-ADAMTS9-AS2(lncRNA-ADAMTS9-AS2)在神经胶质瘤组织中的表达情况及其与胶质瘤病人预后的相关性。方法收集2009年6月至2013年6月手术治疗的原发性神经胶质瘤57例(WHOⅠ～Ⅱ级27例,Ⅲ～Ⅳ级30例)。应用qRT-PCR法分别检测神经胶质瘤组织以及瘤旁正常脑组织lncRNA-ADAMTS9-AS2的表达水平。所有入选病人术后均进行5年随访或直到死亡,根据Kaplan-Meier方法进行生存曲线分析,采用多因素Cox比例风险回归模型分析死亡的风险因素。结果与瘤旁正常脑组织相比,胶质瘤组织lncRNA-ADAMTS9-AS2的表达水平明显降低(P<0.001);而且,高级别(WHOⅢ～Ⅳ级)胶质瘤表达水平明显低于低级别(WHOⅠ～Ⅱ级)胶质瘤(P<0.05)。57例中,lncRNA-ADAMTS9-AS2呈低表达31例(低表达组),高表达26例(高表达组)。57例中,死亡30例,其中低表达组21例(67.7%),高表达组10例(38.5%)。生存曲线分析显示,低表达组病人生存时间较高表达组明显缩短(P<0.05)。多因素Cox比例风险回归模型分析结果显示,lncRNAADAMTS9-AS2低表达是预测胶质瘤病人预后不良的独立风险因素(HR=2.413;95%CI 1.462～4.421;P=0.004)。结论 lncRNAADAMTS9-AS2在神经胶质瘤中表达下调,其可作为胶质瘤病人预后不良的肿瘤标志物。

Objective To investigate the expression of long non-coding RNA ADAMTS9-AS2(lncRNA-ADAMTS9-AS2) in glioma tissues in order to explore the correlativity of its expression level with the clinical prognosis in the patients with gliomas. Methods The levels of ADAMTS9-AS2 expression in 57 cases of glioma tissues of which, 11 belonged in WHO grade Ⅰ 16 in grade Ⅱ, 22 in grade Ⅲ and 8 in grade Ⅳ, and 57 cases of peritumorous normal brain tissues were detected by qRT-PCR, and then the relationship of the expression level of ADAMTS9-AS2 with the clinicopathological factors and prognosis in the patients with gliomas was statistically analyzed. Results The level of ADAMTS9-AS2 expression was significantly lower in the glioma tissues than that in the normal brain tissues(P<0.05). The level of ADAMTS9-AS2 expression was significantly lower in WHO grades Ⅲ~Ⅳ gliomas than that in WHO grades Ⅰ~Ⅱ(P<0.01). The level of ADAMTS9-AS2 expression was significantly higher in the gliomas tissues derived from the patients with Karnofsky performance scale(KPS)≥80 or without the postoperative recurrent gliomas than those in the gliomas tissues derived from the patients with < 80 scores or the patients suffering from the recurrent gliomas after the surgery(P<0.05). Conclusions This study suggested that the down-regulation of ADAMTS9-AS2 expression in the glioma tissues may play a important role in the athogenesis and development of gliomas, and it can be used as a marker of poor prognosis in the patients with gliomas.