不同中药与盐酸恩诺沙星联用对大肠杆菌耐药菌株体外抑菌作用研究

Antibacterial Effect of Different Traditional Chinese Medicines Combined with Enoxacin Hydrochloride on Escherichia coli Resistant Strains in vitro

本试验旨在研究不同中药与盐酸恩诺沙星联用对大肠杆菌耐药菌株的体外抑菌作用。采用微量肉汤法测定临床上一种常用药物盐酸恩诺沙星和不同中药对大肠杆菌耐药菌株及其联合使用的最小抑菌浓度(minimum inhibitory concentration,MIC)。结果发现,单味中药水提物中侧柏叶、金樱子和五味子的抑菌效果较好,其MIC值分别为3.91、3.91和7.81 mg/mL;单味中药的醇提物中,侧柏叶、马齿苋抑菌效果较好,其MIC值分别为7.81和3.91 mg/mL。盐酸恩诺沙星分别与五味子和金樱子水提物联合作用后,对大肠杆菌耐药菌株的MIC值分别降低到0.0078和0.0039 mg/mL;与马齿苋、赤芍、侧柏叶和金樱子的醇提物联合后,对大肠杆菌耐药菌株MIC值均明显降低。盐酸恩诺沙星分别与鱼腥草水提物、赤芍水提物、椿皮水提物、马齿苋醇提物、苍术醇提物、三颗针醇提物、赤芍醇提物、薤白醇提物、拳参醇提物、侧柏叶醇提物、金樱子醇提物和败酱草醇提物联合作用后,对大肠耐药菌株呈现相加或协同抑菌作用,其FIC值分别为0.75、0.62、0.62、0.27、0.75、0.75、0.31、0.62、0.50、0.53、0.28和0.62。初步认为不同中药中含有能有效降低大肠杆菌耐药性的中药,也含有与盐酸恩诺沙星组合,具有协同或相加作用的中药,为寻找有效防治大肠杆菌病的药物组合来对抗多重耐药菌提供了可能。

The purpose of this experiment was to study the in vitro bacteriostasis of different kinds of traditional Chinese herbal medicines combined with enrofloxacin hydrochloride against drug-resistant strains of Escherichia coli ( E.coli ).The minimum inhibitory concentration (MIC) of enrofloxacin hydrochloride (a commonly used drug) and different Chinese herbal medicines against E.coli resistant strains and their combined use was determinated by micro-broth method.The results showed that the antimicrobial effects of Cacumen Biotae,Rosa Laevigata and Schisandra Chinensis Fructus were better in water extracts of single traditional Chinese herbal medicine,and the MIC value were 3.91,3.91 and 7.81 mg/mL,respectively;The antimicrobial effects of Cacumen Biotae and Portulaca Oleracea were better in ethanol extracts of single traditional Chinese herbal medicine,and the MIC value were 7.81 and 3.91 mg/mL,respectively.When enrofloxacin hydrochloride was combined with water extracts of Schisandra Chinensis and Rosa Laevigata ,the MIC value of drug-resistant strains of E.coli reduced to 0.0078 and 0.0039 mg/mL, respectively.When enrofloxacin hydrochloride was combined with ethanol extracts of Portulaca Oleracea,Radix Paeoniae Rubra,Cacumen Biotae and Rosa Laevigata ,the MIC value of drug-resistant strains significantly decreased.When the enrofloxacin hydrochloride was combined with water extract of Houttuynia Herba,Radix Paeoniae Rubra and Ailanthi Cortex ,and alcohol extract of Portulaca Herba,Atractylodis Rhizoma,Berberidis Radix,Paeoniae Radix Rubra,Allii Macrostemi Bulbus,Allii Macrostemonis Bulbus,Platycladi Cacumen,Rosa Laevigatae and Ixeris Denticulata ,it showed adding or synergistic bacteriostatic effect on the E.coli resistant strains,the FIC values were 0.75,0.62,0.62,0.27,0.75,0.75,0.31,0.62,0.50,0.53,0.28 and 0.62,respectively.It was preliminarily believed that there were traditional Chinese herbal medicines that could effectively reduce the drug resistance of E.coli among the different kinds of traditional Chinese herbal medicines,there were also traditional Chinese herbal medicines combined with enrofloxacin hydrochloride,which had synergistic or additive effects.It was possible to find effective drug combinations for the prevention and treatment of E.coli disease to against multi-drug-resistant bacteria.