HPLC法同时检测刺五加叶中原儿茶酸、绿原酸、刺五加苷E、金丝桃苷及槲皮苷的含量

Simultaneous Determination of Protocatechuis acid,Chlorogenic acid, Acanthopanax E, Hyperoside and Quercetin from Acanthopanax Senticosus leaves by HPLC Method

建立HPLC同时定量刺五加叶中原儿茶酸、绿原酸、刺五加苷E、金丝桃苷及槲皮苷五种主要成分的方法。方法:色谱柱:Agilent Extend C18(4.6mm×25mm,5μm),柱温:30℃;流速1 mL/min;进样量:10μL;流动相A:0.3%磷酸-水溶液,流动相B:0.3%磷酸-乙腈;梯度洗脱,洗脱条件:0～3 min,95%A;3～10 min,95%～80%A;10～35 min,80%～70%A;35～40 min,70%～10%A;40～50 min,10%A;紫外检测波长:265 nm。结果:金丝桃苷和槲皮苷在20～240μg/mL,原儿茶酸、绿原酸、刺五加苷E分别在5～60μg/mL、167.2～2010μg/mL及6～72μg/mL下与峰面积呈良好的线性关系;平均加样回收率在99.6%～103.1%,RSD为1.5%～2.7%。结论:本方法简便可靠,适用于刺五加叶中的多成分含量测定。

The paper presents simultaneous determination Of protocatechuis acid, chlorogenic acid, acanthopanax E, hyperoside and quercetin from acanthopanax senticosus leaves by HPLC method as follow. Column method: Agilent Extend C18 ( 4.6mmx25mm, 5μm);Column temperature: 30℃;Flow Rate: 1 mL/min;Injection;10μL;Mobil phase A: 0.3% phosphoric acid solution;Mobil phase B: 0.3% phosphoric acid-acetonitrile;Gradient elution condition: 0-3min, 95%A;3-10min,95-80%A;10-35min, 80-70%A;35-40min,70-10%;40-50min, 10%A;UV detection wavelength;265nm;Result: hyperoside and quercetin contents are 20-240μg/mL;protocatechuis acid, chlorogenic acid and acanthopanax E are 5-60μg/ml, 167.2-2010μg/mL and 6-72μg/mL respectively. There are a good liear relationship with the peak area at 72 μg/mL, the average recovery is 99.6-103.1% and the RSD is 1.5-2.7%.Conclusion: This method is simple and reliable, and is suitiable for the determination ofmulti-component in Acathopanax senticosus leaves .