摘要
为滇重楼和白芨根腐病的防治提供参考依据,对分离自滇重楼和白芨根腐病根部的菌株DB-R进行病原菌形态观察及分子生物学鉴定。结果表明:滇重楼染病后根茎逐渐腐烂,最后整株萎蔫,根茎部腐烂变软;白芨染病后块茎开始出现水渍状腐烂,后块根彻底变黑腐烂;病原菌菌体呈规则圆形,生长迅速,不易被污染,菌丝初期为白色丝状,后变为紫红色;在构建的系统发育树中,获得的菌株DB-R与尖孢镰刀菌(MK156762)聚为一群。引起滇重楼和白芨根腐病的病原菌为尖孢镰刀菌。
In order to provide a reference for the prevention and treatment of Paris polyphylla var. yunnanensis and Bletilla striata of root rot, the pathogen morphology and molecular biology identification of DB-R strain isolated from the roots of P. polyphylla var. yunnanensis and B. striata of root rot were carried out. Results:The rhizome of P. polyphylla var. yunnanensis decayed gradually after being infected, and finally the whole plant wilted and the rhizome decayed and became soft;After B. striata infection, the tuber began to appear water-stained rot. The pathogen body was regular and round, growing rapidly and not easy to be polluted. In the phylogenetic tree constructed, the strain DB-R and fusarium oxysporum (MK156762) were clustered together. Fusarium oxysporum was the cause of the root rot of P. polyphylla var. yunnanensis and B. striata.
作者
赵彩呈
洪英娣
刘丽
张珍荫
熊正勇
伍建榕
ZHAO Caicheng;HONG Yingdi;LIU Li;ZHANG Zhenyin;XIONG Zhengyong;WU Jianrong(Key Laboratory of Biodiversity Conservation in the Southwest Region,Kunming,Yunnan 650224;Key Laboratory of Forest Disaster Warning and Control of Yunnan Province,Southwest Forestry University,Kunming,Yunnan 650224;Forest Protection Station in Yulong County,Lijiang,Yunnan 674100;Forestry Bureau in Ninglang County,Ninglang,Yunnan 674300,China)
出处
《贵州农业科学》
CAS
2019年第5期28-30,35,共4页
Guizhou Agricultural Sciences
基金
国家自然科学基金项目(31360198,31860208)
西南林业大学科技创新基金项目(C17123)
西南林业大学木棉纤维人工林产业化培育省级创新团队项目(2018HC014)
云南省高校基础研究产业化项目(2016CYH14)
三区人才支持项目(201602150,21602128)
关键词
滇重楼
白芨
根腐病
病原菌
尖孢镰刀菌
Paris polyphylla var. yunnanensis
Bletilla striata
root rot
pathogen
fusarium oxysporum
