Caveolin-1、Met蛋白在日光性角化病、鲍温病和皮肤鳞状细胞癌中的表达

Expression of caveolin-1 and Met protein in actinic keratosis, Bowen disease and cutaneous squamous cell carcinoma tissues

目的:检测Caveolin-1、Met蛋白在肿瘤边缘正常皮肤、日光性角化病、鲍温病和皮肤鳞状细胞癌组织中的表达情况。方法:通过免疫组化SP法,对肿瘤边缘正常皮肤、日光性角化病(Actinic keratosis,AK)、鲍温病(Bowen s Disease,BD)、皮肤鳞状细胞癌(cutaneous squamous cell carcinoma,cSCC)标本中Caveolin-1、Met进行检测。结果:Caveolin-1在正常皮肤、AK、BD、SCC中阳性率依次为16.7%、36.8%、52.9%、78.9%,四组间采用Kruskal-wallis H秩和检验,差异具有统计学意义( P < 0.05 )。Met在正常皮肤、AK、BD、SCC中阳性率依次为8.3%、26.3%、58.8%、84.2%,四组间采用Kruskal-wallis H秩和检验, P <0.05,差异具有统计学意义。在cSCC组中,Caveolin-1、Met阳性表达之间呈现正相关( r=0.484,P =0.036)。结论:Caveolin-1、Met在AK、BD和cSCC细胞中表达强度与恶性程度相关,且Caveolin-1、Met可能在cSCC发生、发展中具有协同作用。

Objective: To detect the expressions of Caveolin-1 and Met in adjacent nonmalignant epithelial tissue, actinic keratosis (AK), Bowen disease (BD) and cutaneous squamous cell carcinoma (cSCC) tissues. Methods: The expressions of Caveolin-1 and Met in normal skin tissues, AK, BD and cSCC were detected by immunohistochemistry SP technique. Results: The positive expression rates of Caveolin-1 in normal skin tissues,AK, BD and cSCC were 16.7%, 36.8%, 52.9% and 78.9% respectively and that of Met were 8.3%, 26.3%, 58.8%, 84.2% respectively, with significant differences among four groups by Kruskal-wallis H Test ( P s<0.05). There was a positive correlation between the level Caveolin-1 and Met expression in cutaneous squamous cell carcinoma ( P <0.05). Conclusion: The positive expression rates of Caveolin-1 and Met in four groups showed a gradual increased trend as the grade malignancy of normal skin, AK, BD and cSCC and there may be a synergism between Caveolin-1 and Met in the development of cSCC.