转录因子JunD在HIV-1前病毒DNA转录调控中的作用研究

Role of transcription factor JunD in transcriptional regulation of HIV-1 proviral DNA

人类免疫缺陷病毒1型(HIV-1)潜伏感染的形成与AP-1家族蛋白的转录调节作用密切相关。为研究AP-1家族成员JunD分子在HIV-1潜伏感染形成中的作用,本研究基于可支持HIV-1复制的T细胞系Jurkat,构建敲除JunD和稳定(过)表达JunD的细胞系;拯救HIV-1假病毒并分别感染对照细胞、JunD敲除以及JunD稳定表达的细胞系,通过荧光素酶技术检测HIV-1前病毒DNA转录水平的变化。结果显示,与对照组相比,JunD敲除显著抑制了HIV-1的复制,而过表达JunD则促进了HIV-1的复制。该结果表明,JunD参与了HIV-1前病毒DNA的转录调控,并可能由此影响HIV-1在宿主细胞内的复制。本实验为研究激活HIV-1细胞潜伏感染的靶点提供了实验数据。

HIV-1 latency infection is closely related to the activity of transcription factors from the activating protein-1(AP-1)family.However,whether JunD,a member of AP-1 family,is involved in regulating the transcription of HIV-1 provirus DNA remains unclear.In this study,the JunD knockout and the overexpression Jurkat cell lines were developed.And HIV-1 pseudovirus was used to infect these JunD knockout,overexpressing and control cell lines,respectively.The transcription level of HIV-1 proviral DNA was detected by luciferase assay.The results showed that HIV-1 provirus DNA transcription in the JunD knockout cells was significantly inhibited,compared with that in the control cells,whereas HIV-1 provirus DNA transcription was promoted in the JunD overexpression cells.It suggested that JunD was involved in the activating transcription of HIV-1 provirus DNA and thereby regulating the replication of HIV-1 in host cells.This study uncover a potential target to activate HIV-1 latency infection.