脑心通胶囊肠吸收液清除DPPH自由基的有效成分辨识研究

Effective Components Identification of Naoxintong Capsule Intestinal Absorption Liquid Scavenging DPPH

目的:以肠吸收液-体外活性相结合,并利用拆方重组法研究脑心通胶囊中发挥抗氧化作用的单味或多味中药。方法:应用外翻肠囊法制备肠吸收液,同时拆方或构建重组方,生化试剂盒结合酶标仪检测DPPH自由基的清除率,寻找脑心通胶囊组方中抗氧化作用的单味或多味中药。结果:丹参、黄芪和赤芍的抗氧化作用显著高于脑心通胶囊组,将此三味药组合成三味复方,同时从全方中去除上述三味药,重组成去三味复方,均按原方配比组合,制备肠吸收液,结果显示,三味复方组仍具有较强的抗氧化活性,而去三味组方的抗氧化活性显著降低。当单味药的质量浓度在单味药组、三味复方组及脑心通胶囊组中的质量浓度相同时,三味复方组活性显著低于脑心通胶囊组,与三味药单独作用比较,也明显下降。结论:黄芪、丹参和赤芍是脑心通胶囊抗氧化作用的主要活性成分,但脑心通胶囊抗氧化作用仍是全方的综合作用结果。

Objective: The study aims at finding components in Naoxintong capsule through separation and combination of the prescription with intestinal absorption liquid-activity in vitro as mode of administration. Methods: Naoxintong intestinal absorption liquids of single,combination and total prescription were prepared.DPPH scavenging activity was measured by biochemical tests and a microplate reader.One or more of traditional Chinese medicine in Naoxintong capsule which displayed antioxidant activity were screened through separation and combination of prescription. Results: Antioxidant activity of Radix Salviae Miltiorrhizae,Rstragali Radix and Radix Paeoniae Rubra were stronger than that of Naoxintong capsule.Combined these three kinds of Chinese medicines into one prescription,meanwhile,combined the remained Chinese medicine into the other prescription,the proportion of every ingredient was consistent with original prescription,and then intestinal absorption liquid in each group was prepared.Results showed that antioxidant activity of prescription with the three was the strongest,and antioxidant activity of prescription without the three was quite low.When the concentration of any kind of Chinese medicine in the above three was identical with the concentration in the prescription and single herb,Naoxintong capsule showed the best antioxidant activity,and single herb group took the second place. Conclusion: Radix Salviae Miltiorrhizae,Rstragali Radix and Radix Paeoniae Rubra are the main components exerting antioxidant activities in Naoxintong capsule,but antioxidant activity of Naoxintong capsule is the comprehensive effect of whole herb in the prescription.