ADRB3基因rs9694197多态性与冠心病的关联性研究

Association between ADRB3 rs9694197 gene polymorphism and coronary heart disease

目的探讨β_3肾上腺素能受体(β_3AR)基因(ADRB3)rs9694197多态性与冠状动脉粥样硬化性心脏病(冠心病)患病风险的关联性,为冠心病个体化基因靶向干预治疗提供理论依据。方法本研究采用病例-对照研究,选取2016年1月-2018年12月在新疆医科大学第一附属医院因胸痛症状住院行冠状动脉造影的患者,根据纳入及排除标准,最终入选冠心病患者600例(冠心病组)和非冠心病患者493例(对照组),采用实时荧光定量PCR(Taqman)的方法进行基因分型,运用ABI7900 HT基因检测平台对研究对象ADRB3基因rs9694197位点进行检测。结果 rs9694197三种基因型在病例组人群中的分布频率分别为:AA型占67.1%,AG型占29.2%,GG型占3.7%;在对照组人群中的分布频率分别为:AA型占67.8%,AG型占31.0%, GG型占1.2%,与对照组相比,GG基因型在病例组中的分布频率升高,2组基因型分布差异具有统计学意义(P=0.036)。rs9694197位点A、G等位基因在病例组人群中的分布频率分别为:A等位基因:81.7%,G等位基因:18.3%,在对照组人群中的分布频率分别为:A等位基因:83.3%,G等位基因:16.7%, 2组等位基因分布差异无统计学意义(P=0.354)。多因素非条件Logistic回归分析结果表明,调整性别、年龄,纳入了影响结果的因素后,rs9694197基因多态性仍然和冠心病的发病风险相关,在病例组中,携带GG基因型者患冠心病风险是AA基因型的3.582倍(OR=3.582,95%CI=1.216-10.558,P=0.021)。结论 ADRB3基因rs9694197多态性可能与冠心病相关,GG基因型可能是冠心病发生的危险因素。

Objective To investigate the correlation between the β3 adrenergic receptor(ADRB3) gene polymorphism rs9694197 and the risk of coronary artery atherosclerotic heart disease(coronary heart disease, CHD), in order to provide theoretical basis for individualized gene targeted intervention for coronary heart disease. Methods This study takes up the case-control study strategy, patients with coronary angiography were recruited from January 2016 to December 2018 in the First Affiliated Hospital of Xinjiang Medical University, according to the included and excluded criteria, eventually 600 patients with coronary heart disease(case group) were selected and none CHD were 493 cases(control group) real-time fluorescent quantitative PCR(Taqman) was applied for genotyping, ABI7900 HT genetic testing platform was used to study rs9694197 ADRB3 gene loci. Results The distribution frequencies of the three genotypes of rs9694197 in CHD group were AA type 67.1%, AG type 29.2% and GG type 3.7%, respectively. The distribution frequencies of AA type were 67.8%, AG type 31.0%, and GG type 1.2% in the control group, respectively. The distribution frequencies of GG genotypes in the case group were significantly higher than those in the control group, and the difference between the two groups was statistically significant(P=0.036). The distribution frequencies of A allele and G allele at rs9694197 in the case group were 81.7% and 18.3%, respectively. The distribution frequencies of A allele and G allele in the control group were 83.3% and 16.7%, respectively. No statistically significant difference can be reached between the two groups(P=0.354). Multiariable logistic regression analysis results showed that the adjustment of gender and age, into the factors that affect the result, rs9694197 gene polymorphisms and the risk of coronary heart disease still related, in the case group carrying the GG genotype risk for coronary heart disease is 3.582 times higher than that of AA genotype(OR=3.582, 95% CI=1.216-10.558, P=0.021). Conclusion ADRB3 gene rs9694197 polymorphism may be associated with coronary heart disease, and GG genotype may be a risk factor for coronary heart disease.