摘要
目的:探讨原代哺乳期乳腺细胞中促甲状腺激素(TSH)对钠碘同向转运体(sodium iodide symporter,NIS)的调节作用。方法:在不同浓度TSH 作用下培养小鼠原代哺乳期乳腺细胞,采用实时荧光定量PCR 和Western blot 法检测NIS mRNA和蛋白的表达;Western blot法检测沉默细胞的促甲状腺激素受体(TSHR)后NIS蛋白的表达以及抑制细胞的蛋白激酶A(PKA)后NIS蛋白的表达。结果:小鼠原代哺乳期乳腺细胞TSH高剂量组NIS mRNA表达量最高(P<0.05),NIS蛋白表达差异无统计学意义(P>0.05);沉默TSHR后各组NIS蛋白表达之间差异无统计学意义(P>0.05);PKA抑制组NIS蛋白表达低于TSH组和空白组(P<0.05)。结论:高浓度TSH对小鼠哺乳期乳腺细胞NIS表达具有一定调节作用;PKA可上调NIS的表达。
Objective:To study the regulation mechanism of thyroid stimulating hormone(TSH)to sodium iodide symporter (NIS)in original generation lactating mammary cells of mice. Methods: Original generation lactating mammary cells of mice was cultured under different concentrations of TSH. The expression of NIS mRNA and protein were detected by real-time quantitative PCR and Western blot.The expression of NIS protein after silencing of TSHR of primary cells and after inhibiting protein kinase A (PKA)of primary cells were detected. Results: The highest expression level of NIS mRNA was found in high dose TSH group of original generation lactating mammary cells of mice(P<0.05), and there was no significance in the expression of NIS protein.The expression of NIS protein after inhibiting PKA was lower than that in TSH group and blank group(P<0.05). There was no significant difference in the expression of NIS protein after silencing of TSHR of primary cells(P>0.05). Conclusion: The high concentration of TSH can increase the expression of NIS in original generation lactating mammary cells and PKA plays an important role in increasing the expression of NIS.
作者
史新竹
申红梅
SHI Xinzhu;SHEN Hongmei(Department of Epidemiology,Shenyang Medical College,Shenyang 110034,China;Centre for Endemic Disease Control, Chinese Centre for Disease Control and Prevention,Harbin Medical University)
出处
《沈阳医学院学报》
2019年第3期244-246,283,共4页
Journal of Shenyang Medical College
基金
辽宁省自然科学基金(No.20170540870)
国家自然科学基金(No.81273012)