摘要
提出了一种基于二氨基荧光素类一氧化氮(NO)探针DAF-FM的新型高效NO检测方法。通过纳米金颗粒的添加,显著增强了探针荧光强度,同时NO检测的灵敏度得到了显著的提高。该新型荧光探针可重复使用24次以上,弥补了DAF-FM的单次使用缺陷,实现了一氧化氮的可持续重复检测。
A new and efficient nitric oxide (NO) detection method based on the DAF-FM which is a kind of NO probes was proposed. The incorporation of gold nanoparticles significantly increased the fluorescence intensity of the fluorescent probe after reacting with NO. The new fluorescent probes composed of DAF-FM and gold nanoparticles have dozens of times higher fluorescence intensity than that without gold nanoparticles does. The sensitivity of NO detection has been significantly improved. The new fluorescent probe can be reused for more than 24 times, overcoming the single-use problem of DAF-FM and achieving the sustainable reuse of commercial nitric oxide detection.
作者
王成强
张玲
WANG Chengqiang;ZHANG Ling(School of Optical-Electrical and Computer Engineering,University of Shanghai forScience and Technology,Shanghai 200093,China)
出处
《光学仪器》
2019年第2期13-16,59,共5页
Optical Instruments
基金
国家自然科学基金(61675133、11561121003)
上海市自然科学基金(17ZR1447500)
关键词
一氧化氮(NO)检测
纳米金颗粒
荧光光谱
nitric oxide(NO) detection
gold nanoparticles
fluorescence spectroscopy
