PCR联合HPV检测在宫颈癌筛查中的应用

Application of PCR combined with HPV detection in cervical cancer screening

目的探讨荧光PCR法联合高危型人乳头瘤病毒(HPV)分型核酸测定在宫颈癌癌前病变筛查中的应用及其临床效果。方法选取2016年4月~2018年4月我院收治的宫颈癌患者86例,分为对照组和研究组,对照组患者接受荧光PCR法进行检测筛查,研究组患者在应用荧光PCR法的基础上联合高危型人乳头瘤病毒分型核酸测定进行筛查,同病理诊断结果进行对比。结果研究组检测出的高危型人乳头瘤病毒感染率高达75.6%,研究组检测出的高危型人乳头瘤病毒感染率高于对照组(P<0.05),40~49岁年龄段的人群感染率最高为82.1%,不同年龄阶段高危型人乳头瘤病毒感染的情况不同(P<0.05)。结论荧光PCR法联合高危型人乳头瘤病毒分型核酸测定在宫颈癌癌前病变筛查当中有着非常重要的作用以及对于医学检测具有指导意义,检出率更高,效果理想,临床上应当进一步推广应用。

Objective To explore the application of fluorescent PCR combined with high-risk human papillomavirus typing nucleic acid in the screening of cervical cancer precancerous lesions and its clinical effect. Methods 86 patients with cervical cancer admitted in our hospital from April 2016 to April 2018 were divided into the control group and the study group. The control group was screened by fluorescent PCR. The patients in the study group were treated with the screening of high-risk human papillomavirus typing nucleic acid based on fluorescent PCR. The pathological diagnosis results were compared. Results The high-risk human papillomavirus infection rate detected by the study group was as high as 75.6%. The high-risk human papillomavirus infection rate detected by the study group was higher than that of the control group (P<0.05). The population infection rate of people aged 40-49 years old was as high as 82.1%. And the high-risk human papillomavirus infection was different at different ages(P<0.05). Conclusion Fluorescence PCR combined with high-risk human papillomavirus typing nucleic acid has a very important role in the screening of cervical cancer precancerous lesions and has a guiding role in medical testing. The detection rate is higher and the effect is ideal. It should be further promoted the applied clinically.