摘要
采用国际标准ISO 16604中噬菌体ΦX174的双层琼脂计数方法作为噬菌体ΦX174标准物质定值方法,针对该方法进行了方法验证和协同实验验证以及不确定度分析。结果显示,该方法重复性和复现性均较好,室内重复性相对标准偏差为10. 18%,室间相对标准偏差为14. 58%。经统计分析,双层琼脂计数法相对扩展不确定度为12. 44%,适用于噬菌体ΦX174标准物质的定值,对于人员防护装备防护性能评价具有重要意义。
The double-layer agar counting method of phage ΦX174 in international standard (ISO 16604) was adopted as the calibration method of phage ΦX174 standard substance, and the method was validated by the method and verified by synergistic experiment and the uncertainty analysis. The results showed that the repeatability and reproducibility of the method were good, the relative standard deviation of indoor repeatability was 10.18%, and the relative standard deviation was 14.58%. According to the statistical analysis, the relative expansion uncertainty of the double agar counting method was 12.44%, which was suitable for the determination of the standard substance of phage ΦX174, which was very important for the evaluation of protective performance of personnel protective equipment.
作者
刘思章
隋志伟
张韬
王晶
刘晓夏
王义
张美萍
LIU Si-zhang;SUI Zhi-wei;ZHANG Tao;WANG Jing;LIU Xiao-xia;WANG Yi;ZHANG Mei-ping(College of Life Science, Jilin Agricultural University, Changchun, Jilin 130118, China;National Institute of Metrology, Beijing 100029, China;Wuhan Institute ofVirology,Chinese Academy of Sciences, Wuhan, Hubei 430071, China)
出处
《计量学报》
CSCD
北大核心
2019年第3期511-516,共6页
Acta Metrologica Sinica
基金
国家重点研发计划项目(2017YFF0204602)
国家高技术研究发展计划(2014AA021406)
国家标准物质资源共享平台项目(32-APT1702-9)
关键词
计量学
噬菌体ΦX174
双层琼脂计数法
协同实验验证
metrology
bacteriophage ΦX174
double layer plaque assay method
collaborative experimental verification